Human TPO ELISA Kit

Alternative name
Human TPO ELISA Kit;Human MSA ELISA Kit;Human TDH2A ELISA Kit;Human TPX ELISA Kit;Human thyroid peroxidase ELISA Kit;Human thyroid microsomal antigen ELISA Kit;Human thyroperoxidase ELISA Kit;
Catalog
E002709
species
Human
GeneTPO
SpecificityThis assay has high sensitivity and excellent specificity for detection of TPO-Ab. No significant cross-reactivity or interference between TPO-Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TPO-Ab and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 mIU/l
Intended UseHuman TPO ELISA Kit allows for the in vitro quantitative determination of TPO , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: TPO-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Thyroid peroxidase antigen and an TPO-Ab-HRP conjugate. The assay sample and buffer are incubated together with TPO-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TPO-Ab concentration since TPO-Ab from samples and TPO-Ab-HRP conjugate compete for the Thyroid peroxidase antigen binding site. Since the number of sites is limited, as more sites are occupied by TPO-Ab from the sample, fewer sites are left to bind TPO-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TPO-Ab concentration in each sample is interpolated from this standard curve.
Human Thyroid peroxidase Protein information
Uniprot ID PERT_HUMAN
Uniprot AC Q8TCI9;
UniGene Hs.467554;
GeneID 7173
KEGG hsa:7173;
Human Thyroid peroxidase Protein SEQUENCE
SEQUENCE 933 AA; 102963 MW; F67C5F1A4AEE0B29 CRC64;
MRALAVLSVT LVMACTEAFF PFISRGKELL WGKPEESRVS SVLEESKRLV
DTAMYATMQR NLKKRGILSP AQLLSFSKLP EPTSGVIARA AEIMETSIQA
MKRKVNLKTQ QSQHPTDALS EDLLSIIANM SGCLPYMLPP KCPNTCLANK
YRPITGACNN RDHPRWGASN TALARWLPPV YEDGFSQPRG WNPGFLYNGF
PLPPVREVTR HVIQVSNEVV TDDDRYSDLL MAWGQYIDHD IAFTPQSTSK
AAFGGGADCQ MTCENQNPCF PIQLPEEARP AAGTACLPFY RSSAACGTGD
QGALFGNLST ANPRQQMNGL TSFLDASTVY GSSPALERQL RNWTSAEGLL
RVHARLRDSG RAYLPFVPPR APAACAPEPG IPGETRGPCF LAGDGRASEV
PSLTALHTLW LREHNRLAAA LKALNAHWSA DAVYQEARKV VGALHQIITL
RDYIPRILGP EAFQQYVGPY EGYDSTANPT VSNVFSTAAF RFGHATIHPL
VRRLDASFQE HPDLPGLWLH QAFFSPWTLL RGGGLDPLIR GLLARPAKLQ
VQDQLMNEEL TERLFVLSNS STLDLASINL QRGRDHGLPG YNEWREFCGL
PRLETPADLS TAIASRSVAD KILDLYKHPD NIDVWLGGLA ENFLPRARTG
PLFACLIGKQ MKALRDGDWF WWENSHVFTD AQRRELEKHS LSRVICDNTG
LTRVPMDAFQ VGKFPEDFES CDSITGMNLE AWRETFPQDD KCGFPESVEN
GDFVHCEESG RRVLVYSCRH GYELQGREQL TCTQEGWDFQ PPLCKDVNEC
ADGAHPPCHA SARCRNTKGG FQCLCADPYE LGDDGRTCVD SGRLPRVTWI
SMSLAALLIG GFAGLTSTVI CRWTRTGTKS TLPISETGGG TPELRCGKHQ
AVGTSPQRAA AQDSEQESAG MEGRDTHRLP RAL
UCSC uc002qwr.4; human. [P07202-1];

Category

Human TPO ELISA Kit

Human TPO ELISA Kit;Human MSA ELISA Kit;Human TDH2A ELISA Kit;Human TPX ELISA Kit;Human thyroid peroxidase ELISA Kit;Human thyroid microsomal antigen ELISA Kit;Human thyroperoxidase ELISA Kit;