Human Abeta ELISA Kit

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  • Alternative name

    Amyloid beta A4 protein ELISA KIT; ABPP ELISA KIT; APPI ELISA KIT; APP ELISA KIT; Alzheimer disease amyloid protein ELISA KIT; Cerebral vascular amyloid peptide ELISA KIT; CVAP ELISA KIT; PreA4 ELISA KIT; Protease nexin-II ELISA KIT; PN-IICleaved into the following 14 chains:N-APPSoluble APP-alpha ELISA KIT; S-APP-alphaSoluble APP-beta ELISA KIT; S-APP-betaC99Beta-amyloid protein 42 ELISA KIT; Alternative name(s): ELISA KIT; Beta-APP42Beta-amyloid protein 40 ELISA KIT; Alternative name(s): ELISA KIT; Beta-APP40C83P3(42)P3(40)C80Gamma-secretase C-terminal fragment 59 ELISA KIT; Alternative name(s): ELISA KIT; Amyloid intracellular domain 59 ELISA KIT; AICD-59 ELISA KIT; AID(59) ELISA KIT; Gamma-CTF(59)Gamma-secretase C-terminal fragment 57 ELISA KIT; Alternative name(s): ELISA KIT; Amyloid intracellular domain 57 ELISA KIT; AICD-57 ELISA KIT; AID(57) ELISA KIT; Gamma-CTF(57)Gamma-secretase C-terminal fragment 50 ELISA KIT; Alternative name(s): ELISA KIT; Amyloid intracellular domain 50 ELISA KIT; AICD-50 ELISA KIT; AID(50) ELISA KIT; Gamma-CTF(50)APP ELISA KIT; A4 ELISA KIT; AD1 ELISA KIT; APP ELISA KIT; CVAP ELISA KIT; PN-II ELISA KIT; S-APP-alpha ELISA KIT; S-APP-beta ELISA KIT; AICD-59 ELISA KIT; AID(59) ELISA KIT; AICD-57 ELISA KIT; AID(57) ELISA KIT; AICD-50 ELISA KIT; AID(50) ELISA KIT

  • Catalog
    E038618
  • species
    Human
  • GeneAbeta
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Abeta. No significant cross-reactivity or interference between Abeta and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Abeta and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman Abeta ELISA Kit allows for the in vitro quantitative determination of Abeta , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyHuman ELISA Kit
  • Product Description
    specifical
    Principle of the Assay: Abeta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Abeta antibody and an Abeta-HRP conjugate. The assay sample and buffer are incubated together with Abeta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Abeta concentration since Abeta from samples and Abeta-HRP conjugate compete for the anti-Abeta antibody binding site. Since the number of sites is limited, as more sites are occupied by Abeta from the sample, fewer sites are left to bind Abeta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Abeta concentration in each sample is interpolated from this standard curve.



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