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Catalog
C001147
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Alternative name
Nucleotide-binding oligomerization domain-containing protein 2 CLIA KIT; Caspase recruitment domain-containing protein 15 CLIA KIT; Inflammatory bowel disease protein 1NOD2 CLIA KIT; CARD15 CLIA KIT; IBD1 CLIA KIT
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GeneNOD2
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of NOD2. No significant cross-reactivity or interference between NOD2 and analogues was observed.
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SamplesHuman tissue homogenates, cell lysates and other biological fluids.
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Detection Range41.2-30,000pg/mL. The standard curve concentrations used for the CLIA's were 30,000pg/mL, 10,000pg/mL, 3,333.3pg/mL, 1,111.1pg/mL, 370.4pg/mL, 123.5pg/mL, 41.2pg/mL.
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Sensitivity17.4pg/mL.
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Product Description specificalIntended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of NOD2 in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to NOD2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NOD2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the NOD2 level in the sample or standard.
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