-
Catalog
C000121
-
Alternative name
Vascular cell adhesion protein 1 CLIA KIT; INCAM-100 CLIA KIT; CD_antigen: CD106VCAM1 CLIA KIT; L1CAM CLIA KIT; V-CAM 1 CLIA KIT; VCAM-1 CLIA KIT
-
GeneVCAM1
-
Species ReactivityMouse
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of Vascular Cell Adhesion Molecule 1 (VCAM1).
No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 (VCAM1) and analogues was observed.
-
SamplesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
-
Detection Range68.59-50000pg/mL
-
PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
-
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Vascular Cell Adhesion Molecule 1 (VCAM1) level in the sample or standard.
-