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Catalog
C001741
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Alternative name
Interleukin-17A CLIA KIT; Cytotoxic T-lymphocyte-associated antigen 8 CLIA KIT; CTLA-8IL17A CLIA KIT; CTLA8 CLIA KIT; IL17 CLIA KIT; IL-17 CLIA KIT; IL-17A CLIA KIT; CTLA-8 CLIA KIT
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GeneIL17
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of IL17. No significant cross-reactivity or interference between IL17 and analogues was observed.
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Detection Range2.74-2,000pg/mL. The standard curve concentrations used for the CLIA's were 2,000pg/mL, 666.67pg/mL, 222.22pg/mL, 74.07pg/mL, 24.69pg/mL, 8.23pg/mL, 2.74pg/mL.
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SensitivityThe minimum detectable dose of IL17 is typically less than 1.14pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
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Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL17. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IL17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the IL17 level in the sample or standard.
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