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Catalog
C002100
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Alternative name
Hypoxia-inducible factor 1-alpha CLIA KIT; ARNT-interacting protein CLIA KIT; Basic-helix-loop-helix-PAS protein MOP1 CLIA KIT; Class E basic helix-loop-helix protein 78 CLIA KIT; bHLHe78 CLIA KIT; Member of PAS protein 1 CLIA KIT; PAS domain-containing protein 8HIF1A CLIA KIT; BHLHE78 CLIA KIT; MOP1 CLIA KIT; PASD8 CLIA KIT; HIF-1-alpha CLIA KIT; HIF1-alpha CLIA KIT; bHLHe78 CLIA KIT
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GeneHIF1a
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 1 Alpha (HIF1a).
No significant cross-reactivity or interference between Hypoxia Inducible Factor 1 Alpha (HIF1a) and analogues was observed.
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SamplesSerum, plasma and other biological fluids
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Detection Range13.72-10000pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 4.93pg/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hypoxia Inducible Factor 1 Alpha (HIF1a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hypoxia Inducible Factor 1 Alpha (HIF1a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hypoxia Inducible Factor 1 Alpha (HIF1a) level in the sample or standard.
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