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Catalog
C002177
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Alternative name
Hypoxia-inducible factor 1-alpha CLIA KIT; ARNT-interacting protein CLIA KIT; Basic-helix-loop-helix-PAS protein MOP1 CLIA KIT; Class E basic helix-loop-helix protein 78 CLIA KIT; bHLHe78 CLIA KIT; Member of PAS protein 1 CLIA KIT; PAS domain-containing protein 8HIF1A CLIA KIT; BHLHE78 CLIA KIT; MOP1 CLIA KIT; PASD8 CLIA KIT; HIF-1-alpha CLIA KIT; HIF1-alpha CLIA KIT; bHLHe78 CLIA KIT
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GeneHIF-1a
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Species ReactivityHuman
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SpecificityThis kit recognizes natural and recombinant HIF-1alpha. No significant cross-reactivity or interference between HIF-1alpha and analogues was observed.
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SamplesSerum, plasma and other biological fluids.
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Detection Range31.25-2000pg/mL
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Sensitivity18.75pg/mL
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Product Description specificalIntended Uses: This CLIA kit applies to the in vitro quantitative determination of HIF-1alpha concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to HIF-1alpha. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HIF-1alpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HIF-1alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of HIF-1alpha. You can calculate the concentration of HIF-1alpha in the samples by comparing the RLU value of the samples to the standard curve.
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