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Catalog
C002178
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Alternative name
Hypoxia-inducible factor 1-alpha CLIA KIT; ARNT-interacting protein CLIA KIT; Basic-helix-loop-helix-PAS protein MOP1 CLIA KIT; Class E basic helix-loop-helix protein 78 CLIA KIT; bHLHe78 CLIA KIT; Member of PAS protein 1 CLIA KIT; PAS domain-containing protein 8HIF1A CLIA KIT; BHLHE78 CLIA KIT; MOP1 CLIA KIT; PASD8 CLIA KIT; HIF-1-alpha CLIA KIT; HIF1-alpha CLIA KIT; bHLHe78 CLIA KIT
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GeneHIF-1a
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Species ReactivityRat
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Detection Range7.813~500pg/mL
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Sensitivity4.688pg/mL
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Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HIF-1alpha. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HIF-1alpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HIF-1alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat HIF-1alpha. You can calculate the concentration of Rat HIF-1alpha in the samples by comparing the RLU value of the samples to the standard curve.
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