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Catalog
C002211
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Alternative name
Proheparin-binding EGF-like growth factor CLIA KIT; Hbegf CLIA KIT; Dtr CLIA KIT; Hegfl CLIA KIT; HB-EGF CLIA KIT; HBEGF CLIA KIT
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GeneHBEGF
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Species ReactivityMouse
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF).
No significant cross-reactivity or interference between Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) and analogues was observed.
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SamplesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
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Detection Range1.37-1000pg/mL
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SensitivityThe minimum detectable dose of this kit is typically less than 0.64pg/mL.
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PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) level in the sample or standard.
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