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Catalog
C002229
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GeneHbA1c
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Species ReactivityRat
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SpecificityThis kit recognizes natural and recombinant HbA1c. No significant cross-reactivity or interference between HbA1c and analogues was observed.
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SamplesSerum, plasma and other biological fluids.
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Detection Range0.313-20ng/mL
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SensitivityThe minimum detectable dose of HbA1c is 0.188ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
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Product Description specificalPrinciple of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to HbA1c. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HbA1c and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HbA1c, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of HbA1c. You can calculate the concentration of HbA1c in the samples by comparing the RLU value of the samples to the standard curve.
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