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Catalog
C002797
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Alternative name
Serine/threonine-protein kinase/endoribonuclease IRE1 CLIA KIT; Endoplasmic reticulum-to-nucleus signaling 1 CLIA KIT; Inositol-requiring protein 1 CLIA KIT; hIRE1p CLIA KIT; Ire1-alpha CLIA KIT; IRE1aERN1 CLIA KIT; hIRE1p CLIA KIT; IRE1a CLIA KIT
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GeneERN1
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Species ReactivityRat
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Detection Range15.625~1000pg/mL
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Sensitivity9.375pg/mL
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Product Description specificalThe micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ERN1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ERN1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ERN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ERN1. You can calculate the concentration of Rat ERN1 in the samples by comparing the RLU value of the samples to the standard curve.
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