-
Catalog
C002848
-
GeneECF
-
Species ReactivityBovine
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of Eosinophil Chemotactic Factor (ECF).
No significant cross-reactivity or interference between Eosinophil Chemotactic Factor (ECF) and analogues was observed.
-
SamplesSerum, plasma and other biological fluids
-
Detection Range5.49-4000pg/mL
-
SensitivityThe minimum detectable dose of this kit is typically less than 2.48pg/mL.
-
PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
-
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Eosinophil Chemotactic Factor (ECF) level in the sample or standard.
-