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Catalog
C002917
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Alternative name
Desmoplakin CLIA KIT; Desmoplakindesmoplakin CLIA KIT;
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GeneDSP
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Species ReactivityHuman
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SpecificityThis kit recognizes natural and recombinant DSP. No significant cross-reactivity or interference between DSP and analogues was observed.
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SamplesSerum, plasma and other biological fluids.
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Detection Range62.5-4000pg/mL
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SensitivityThe minimum detectable dose of DSP is 37.5pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
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Product Description specificalPrinciple of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to DSP. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DSP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DSP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of DSP. You can calculate the concentration of DSP in the samples by comparing the RLU value of the samples to the standard curve.
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