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Catalog
C003182
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Alternative name
Collagen alpha-1(IV) chain CLIA KIT; Cg25C CLIA KIT; DCg1 CLIA KIT
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GeneCOL4
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of COL4. No significant cross-reactivity or interference between COL4 and analogues was observed.
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SamplesHuman serum, plasma, tissue homogenates and other biological fluids.
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Detection Range0.14-100ng/mL. The standard curve concentrations used for the CLIA's were 100ng/mL, 33.33ng/mL, 11.11ng/mL, 3.70ng/mL, 1.23ng/mL, 0.41ng/mL, 0.14ng/mL.
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Sensitivity0.07ng/mL.
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Product Description specificalIntended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of COL4 in human serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the COL4 level in the sample or standard.
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