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Catalog
C003748
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Alternative name
Apelin CLIA KIT; APJ endogenous ligandAPLN CLIA KIT; APEL CLIA KIT
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GeneAPLN
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Species ReactivityHuman
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Detection Range62.5~4000pg/mL
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Sensitivity37.5pg/mL
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Product Description specificalThis CLIA kit uses Competitive-CLiA as the method. The microtiter plate provided in this kit has been pre-coated with Human APLN. During the reaction, Human APLN in the sample or standard competes with a fixed amount of Human APLN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human APLN. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The Relative light unit(RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is negatively associated with the concentration of Human APLN. You can calculate the concentration of Human APLN in the samples by comparing the RLU of the samples to the standard curve.
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