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Catalog
C003774
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Alternative name
Natriuretic peptides A CLIA KIT; CDD-ANF CLIA KIT; Cardiodilatin CLIA KIT; CDDNPPA CLIA KIT; ANP CLIA KIT; PND CLIA KIT; CDD CLIA KIT; CDP CLIA KIT; ANF CLIA KIT; ANP CLIA KIT
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GeneANP
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Species ReactivityHuman
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SpecificityThis kit recognizes natural and recombinant Human ANP. No significant cross-reactivity or interference between Human ANP and analogues was observed.
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SamplesSerum, plasma and other biological fluids.
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Detection Range7.813-500pg/mL
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SensitivityThe minimum detectable dose of Human ANP is 4.688pg/mL
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Product Description specificalIntended Uses||This CLIA kit applies to the in vitro quantitative determination of Human ANP concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This CLIA kit uses Competitive-CLIA as the method. The microtiter plate provided in this kit has been pre-coated with Human ANP. During the reaction, Human ANP in the sample or standard competes with a fixed amount of Human ANP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human ANP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The Relative light unit (RLU) valueis measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is negatively associated with the concentration of Human ANP. You can calculate the concentration of Human ANP in the samples by comparing the RLU of the samples to the standard curve.
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