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Catalog
C003890
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Alternative name
Serum albumin CLIA KIT; ALB CLIA KIT;
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GeneALB
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Species ReactivityHuman
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SpecificityThis assay has high sensitivity and excellent specificity for detection of ALB. No significant cross-reactivity or interference between ALB and analogues was observed.
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Detection Range1.56-400mug/mL. The standard curve concentrations used for the CLIA's were 400mug/mL, 100mug/mL, 25mug/mL, 6.25mug/mL, 1.56mug/mL.
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SensitivityThe minimum detectable dose of ALB is typically less than 0.52mug/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard subtracted by their two standard deviations.
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Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to ALB. A competitive inhibition reaction is launched between biotin labeled ALB and unlabeled ALB (Standards or samples) with the pre-coated antibody specific to ALB. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of ALB in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the ALB level in the sample or standard.
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