Human CUL4A ELISA Kit

Alternative name
Human CUL-4A ELISA Kit;Human cullin 4A ELISA Kit;Human cullin-4A ELISA Kit;
Catalog
E010384
species
Human
GeneCUL4A
SpecificityThis assay has high sensitivity and excellent specificity for detection of CUL4A. No significant cross-reactivity or interference between CUL4A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CUL4A and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CUL4A ELISA Kit allows for the in vitro quantitative determination of CUL4A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: CUL4A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CUL4A antibody and an CUL4A-HRP conjugate. The assay sample and buffer are incubated together with CUL4A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CUL4A concentration since CUL4A from samples and CUL4A-HRP conjugate compete for the anti-CUL4A antibody binding site. Since the number of sites is limited, as more sites are occupied by CUL4A from the sample, fewer sites are left to bind CUL4A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CUL4A concentration in each sample is interpolated from this standard curve.
Human Cullin-4A Protein information
Uniprot ID CUL4A_HUMAN
Uniprot AC Q13619; A2A2W2; O75834; Q589T6; Q5TC62; Q6UP08; Q9UP17;
UniGene Hs.339735;
GeneID 8451
KEGG hsa:8451;
Human Cullin-4A Protein SEQUENCE
SEQUENCE 759 AA; 87680 MW; 3C4C6A1BBD94D51B CRC64;
MADEAPRKGS FSALVGRTNG LTKPAALAAA PAKPGGAGGS KKLVIKNFRD
RPRLPDNYTQ DTWRKLHEAV RAVQSSTSIR YNLEELYQAV ENLCSHKVSP
MLYKQLRQAC EDHVQAQILP FREDSLDSVL FLKKINTCWQ DHCRQMIMIR
SIFLFLDRTY VLQNSTLPSI WDMGLELFRT HIISDKMVQS KTIDGILLLI
ERERSGEAVD RSLLRSLLGM LSDLQVYKDS FELKFLEETN CLYAAEGQRL
MQEREVPEYL NHVSKRLEEE GDRVITYLDH STQKPLIACV EKQLLGEHLT
AILQKGLDHL LDENRVPDLA QMYQLFSRVR GGQQALLQHW SEYIKTFGTA
IVINPEKDKD MVQDLLDFKD KVDHVIEVCF QKNERFVNLM KESFETFINK
RPNKPAELIA KHVDSKLRAG NKEATDEELE RTLDKIMILF RFIHGKDVFE
AFYKKDLAKR LLVGKSASVD AEKSMLSKLK HECGAAFTSK LEGMFKDMEL
SKDIMVHFKQ HMQNQSDSGP IDLTVNILTM GYWPTYTPME VHLTPEMIKL
QEVFKAFYLG KHSGRKLQWQ TTLGHAVLKA EFKEGKKEFQ VSLFQTLVLL
MFNEGDGFSF EEIKMATGIE DSELRRTLQS LACGKARVLI KSPKGKEVED
GDKFIFNGEF KHKLFRIKIN QIQMKETVEE QVSTTERVFQ DRQYQIDAAI
VRIMKMRKTL GHNLLVSELY NQLKFPVKPG DLKKRIESLI DRDYMERDKD
NPNQYHYVA
UCSC uc021rmu.2; human. [Q13619-1];

Category

Human CUL4A ELISA Kit

Human CUL-4A ELISA Kit;Human cullin 4A ELISA Kit;Human cullin-4A ELISA Kit;