Human CREB3L3 ELISA Kit

Alternative name
Human cAMP-responsive element-binding protein 3-like protein 3 ELISA Kit;Human Transcription factor CREB-H ELISA Kit;Human CREBH ELISA Kit;Human CREB-H ELISA Kit;Human HYST1481 ELISA Kit;Human cAMP responsive element binding protein 3 like 3 ELISA Kit;Human cyclic AMP-responsive element-binding protein 3-like protein 3 ELISA Kit;Human CREB/ATF family transcription factor ELISA Kit;
Catalog
E010496
species
Human
GeneCREB3L3
SpecificityThis assay has high sensitivity and excellent specificity for detection of CREB3L3. No significant cross-reactivity or interference between CREB3L3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CREB3L3 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CREB3L3 ELISA Kit allows for the in vitro quantitative determination of CREB3L3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalIntended Uses: This CREB3L3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CREB3L3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CREB3L3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CREB3L3 antibody and an CREB3L3-HRP conjugate. The assay sample and buffer are incubated together with CREB3L3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CREB3L3 concentration since CREB3L3 from samples and CREB3L3-HRP conjugate compete for the anti-CREB3L3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CREB3L3 from the sample, fewer sites are left to bind CREB3L3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CREB3L3 concentration in each sample is interpolated from this standard curve.
Human Processed cyclic AMP-responsive element-binding protein 3-like protein 3 Protein information
Uniprot ID CR3L3_HUMAN
Uniprot AC Q68CJ9; B2R7S6; B7ZL69; M0QYW7; Q6ZMC5; Q96TB9;
UniGene Hs.247744;
GeneID 84699
KEGG hsa:84699;
Human Processed cyclic AMP-responsive element-binding protein 3-like protein 3 Protein SEQUENCE
SEQUENCE 461 AA; 49077 MW; C40F5AA626661F80 CRC64;
MNTDLAAGKM ASAACSMDPI DSFELLDLLF DRQDGILRHV ELGEGWGHVK
DQQVLPNPDS DDFLSSILGS GDSLPSSPLW SPEGSDSGIS EDLPSDPQDT
PPRSGPATSP AGCHPAQPGK GPCLSYHPGN SCSTTTPGPV IQVPEASVTI
DLEMWSPGGR ICAEKPADPV DLSPRCNLTV KDLLLSGSSG DLQQHHLGAS
YLLRPGAGHC QELVLTEDEK KLLAKEGITL PTQLPLTKYE ERVLKKIRRK
IRNKQSAQES RKKKKEYIDG LETRMSACTA QNQELQRKVL HLEKQNLSLL
EQLKKLQAIV VQSTSKSAQT GTCVAVLLLS FALIILPSIS PFGPNKTESP
GDFAPVRVFS RTLHNDAASR VAADAVPGSE APGPRPEADT TREESPGSPG
ADWGFQDTAN LTNSTEELDN ATLVLRNATE GLGQVALLDW VAPGPSTGSG
RAGLEAAGDE L
UCSC uc002lzl.4; human. [Q68CJ9-1];

Category

Human CREB3L3 ELISA Kit