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Human CCNB2 ELISA Kit

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  • Alternative name

    Human HsT17299 ELISA Kit;Human cyclin B2 ELISA Kit;Human G2/mitotic-specific cyclin-B2 ELISA Kit;
  • Catalog
    E010527
  • species
    Human
  • GeneCCNB2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CCNB2. No significant cross-reactivity or interference between CCNB2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.054ng/mL.
  • Intended UseHuman CCNB2 ELISA Kit allows for the in vitro quantitative determination of CCNB2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CCNB2 in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCNB2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CCNB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CCNB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CCNB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human G2/mitotic-specific cyclin-B2 Protein information
  • Uniprot ID CCNB2_HUMAN
  • Uniprot AC O95067; B3KM93; Q6FI99;
  • UniGene Hs.194698;
  • GeneID 9133
  • KEGG hsa:9133;
  • Human G2/mitotic-specific cyclin-B2 Protein SEQUENCE

  • SEQUENCE 398 AA; 45282 MW; 874466E1DD68A4C4 CRC64;

    MALLRRPTVS SDLENIDTGV NSKVKSHVTI RRTVLEEIGN RVTTRAAQVA

    KKAQNTKVPV QPTKTTNVNK QLKPTASVKP VQMEKLAPKG PSPTPEDVSM

    KEENLCQAFS DALLCKIEDI DNEDWENPQL CSDYVKDIYQ YLRQLEVLQS

    INPHFLDGRD INGRMRAILV DWLVQVHSKF RLLQETLYMC VGIMDRFLQV

    QPVSRKKLQL VGITALLLAS KYEEMFSPNI EDFVYITDNA YTSSQIREME

    TLILKELKFE LGRPLPLHFL RRASKAGEVD VEQHTLAKYL MELTLIDYDM

    VHYHPSKVAA AASCLSQKVL GQGKWNLKQQ YYTGYTENEV LEVMQHMAKN

    VVKVNENLTK FIAIKNKYAS SKLLKISMIP QLNSKAVKDL ASPLIGRS

  • UCSC uc002afz.4; human.;



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