Human ADRA1A ELISA Kit

Alternative name
Human alpha-1A adrenoreceptor ELISA Kit;Human alpha-1A adrenoceptor ELISA Kit;Human alpha-1C adrenergic receptor ELISA Kit;Human Alpha-adrenergic receptor 1c ELISA Kit;Human ADRA1C ELISA Kit;Human ADRA1L1 ELISA Kit;Human ALPHA1AAR ELISA Kit;Human adrenoceptor alpha 1A ELISA Kit;Human alpha-1A adrenergic receptor ELISA Kit;Human G protein coupled receptor ELISA Kit;Human adrenergic, alpha-1A-, receptor ELISA Kit;
Catalog
E001055
species
Human
GeneADRA1A
SpecificityThis assay has high sensitivity and excellent specificity for detection of Adrenergic Receptor Alpha 1A (ADRa1A). No significant cross-reactivity or interference between Adrenergic Receptor Alpha 1A (ADRa1A) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.114ng/mL.
Intended UseHuman ADRA1A ELISA Kit allows for the in vitro quantitative determination of ADRA1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adrenergic Receptor Alpha 1A (ADRa1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adrenergic Receptor Alpha 1A (ADRa1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adrenergic Receptor Alpha 1A (ADRa1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adrenergic Receptor Alpha 1A (ADRa1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Alpha-1A adrenergic receptor Protein information
Uniprot ID ADA1A_HUMAN
Uniprot AC Q6RUJ8; Q6RUJ9; Q96RE8; Q9UD63; Q9UD67;
UniGene Hs.52931; Hs.709175;
GeneID 148
KEGG hsa:148;
Human Alpha-1A adrenergic receptor Protein SEQUENCE
SEQUENCE 466 AA; 51487 MW; 1A50487531DECDF0 CRC64;
MVFLSGNASD SSNCTQPPAP VNISKAILLG VILGGLILFG VLGNILVILS
VACHRHLHSV THYYIVNLAV ADLLLTSTVL PFSAIFEVLG YWAFGRVFCN
IWAAVDVLCC TASIMGLCII SIDRYIGVSY PLRYPTIVTQ RRGLMALLCV
WALSLVISIG PLFGWRQPAP EDETICQINE EPGYVLFSAL GSFYLPLAII
LVMYCRVYVV AKRESRGLKS GLKTDKSDSE QVTLRIHRKN APAGGSGMAS
AKTKTHFSVR LLKFSREKKA AKTLGIVVGC FVLCWLPFFL VMPIGSFFPD
FKPSETVFKI VFWLGYLNSC INPIIYPCSS QEFKKAFQNV LRIQCLCRKQ
SSKHALGYTL HPPSQAVEGQ HKDMVRIPVG SRETFYRISK TDGVCEWKFF
SSMPRGSARI TVSKDQSSCT TARVRSKSFL QVCCCVGPST PSLDKNHQVP
TIKVHTISLS ENGEEV
UCSC uc003xfc.1; human. [P35348-1];

Category

Human ADRA1A ELISA Kit