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Human CDKN1B ELISA Kit

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  • Alternative name

    Human Cyclin-dependent kinase inhibitor p27 ELISA Kit;Human P27KIP1 ELISA Kit;Human KIP1 ELISA Kit;Human CDKN4 ELISA Kit;Human MEN1B ELISA Kit;Human MEN4 ELISA Kit;Human cyclin dependent kinase inhibitor 1B ELISA Kit;Human cyclin-dependent kinase inhibitor 1B ELISA Kit;Human cyclin-dependent kinase inhibitor 1B (p27, Kip1) ELISA Kit;
  • Catalog
    E010594
  • species
    Human
  • GeneCDKN1B
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CDKN1B. No significant cross-reactivity or interference between CDKN1B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDKN1B and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman CDKN1B ELISA Kit allows for the in vitro quantitative determination of CDKN1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Intended Uses: This CDKN1B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CDKN1B. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CDKN1B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDKN1B antibody and an CDKN1B -HRP conjugate. The assay sample and buffer are incubated together with CDKN1B -HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDKN1B concentration since CDKN1B from samples and CDKN1B -HRP conjugate compete for the anti-CDKN1B antibody binding site. Since the number of sites is limited, as more sites are occupied by CDKN1B from the sample, fewer sites are left to bind CDKN1B -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDKN1B concentration in each sample is interpolated from this standard curve.
  • Human Cyclin-dependent kinase inhibitor 1B Protein information
  • Uniprot ID CDN1B_HUMAN
  • Uniprot AC P46527; Q16307; Q5U0H2; Q9BUS6;
  • UniGene Hs.238990;
  • GeneID 1027
  • KEGG hsa:1027;
  • Human Cyclin-dependent kinase inhibitor 1B Protein SEQUENCE

  • SEQUENCE 198 AA; 22073 MW; 1118D58901CDF3FC CRC64;

    MSNVRVSNGS PSLERMDARQ AEHPKPSACR NLFGPVDHEE LTRDLEKHCR

    DMEEASQRKW NFDFQNHKPL EGKYEWQEVE KGSLPEFYYR PPRPPKGACK

    VPAQESQDVS GSRPAAPLIG APANSEDTHL VDPKTDPSDS QTGLAEQCAG

    IRKRPATDDS STQNKRANRT EENVSDGSPN AGSVEQTPKK PGLRRRQT




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