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Human CYBB ELISA Kit

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  • Alternative name

    Human CGD91-phox ELISA Kit;Human cytochrome b(558) subunit beta ELISA Kit;Human cytochrome b558 subunit beta ELISA Kit;Human heme-binding membrane glycoprotein gp91phox ELISA Kit;Human NADPH oxidase 2 ELISA Kit;Human neutrophil cytochrome b 91 kDa polypeptide ELISA Kit;Human superoxide-generating NADPH oxidase heavy chain subunit ELISA Kit;Human GP91-1 ELISA Kit;Human GP91-PHOX ELISA Kit;Human p22 phagocyte B-cytochrome ELISA Kit;Human NOX2 ELISA Kit;Human AMCBX2 ELISA Kit;Human CGD ELISA Kit;Human GP91PHOX ELISA Kit;Human IMD34 ELISA Kit;Human p91-PHOX ELISA Kit;Human cytochrome b-245 beta chain ELISA Kit;Human cytochrome b-245 heavy chain ELISA Kit;Human cytochrome b-245 beta polypeptide ELISA Kit;
  • Catalog
    E010926
  • species
    Human
  • GeneCYBB
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome b-245 Beta Polypeptide (CYBb). No significant cross-reactivity or interference between Cytochrome b-245 Beta Polypeptide (CYBb) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman CYBB ELISA Kit allows for the in vitro quantitative determination of CYBB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome b-245 Beta Polypeptide (CYBb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome b-245 Beta Polypeptide (CYBb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome b-245 Beta Polypeptide (CYBb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome b-245 Beta Polypeptide (CYBb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cytochrome b-245 heavy chain Protein information
  • Uniprot ID CY24B_HUMAN
  • Uniprot AC P04839; A8K138; Q2PP16;
  • UniGene Hs.292356;
  • GeneID 1536
  • KEGG hsa:1536;
  • Human Cytochrome b-245 heavy chain Protein SEQUENCE

  • SEQUENCE 570 AA; 65336 MW; 7E84051BD4000CE3 CRC64;

    MGNWAVNEGL SIFVILVWLG LNVFLFVWYY RVYDIPPKFF YTRKLLGSAL

    ALARAPAACL NFNCMLILLP VCRNLLSFLR GSSACCSTRV RRQLDRNLTF

    HKMVAWMIAL HSAIHTIAHL FNVEWCVNAR VNNSDPYSVA LSELGDRQNE

    SYLNFARKRI KNPEGGLYLA VTLLAGITGV VITLCLILII TSSTKTIRRS

    YFEVFWYTHH LFVIFFIGLA IHGAERIVRG QTAESLAVHN ITVCEQKISE

    WGKIKECPIP QFAGNPPMTW KWIVGPMFLY LCERLVRFWR SQQKVVITKV

    VTHPFKTIEL QMKKKGFKME VGQYIFVKCP KVSKLEWHPF TLTSAPEEDF

    FSIHIRIVGD WTEGLFNACG CDKQEFQDAW KLPKIAVDGP FGTASEDVFS

    YEVVMLVGAG IGVTPFASIL KSVWYKYCNN ATNLKLKKIY FYWLCRDTHA

    FEWFADLLQL LESQMQERNN AGFLSYNIYL TGWDESQANH FAVHHDEEKD

    VITGLKQKTL YGRPNWDNEF KTIASQHPNT RIGVFLCGPE ALAETLSKQS

    ISNSESGPRG VHFIFNKENF

  • UCSC uc004ddr.3; human.;



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