Human CYP1B1 ELISA Kit

Alternative name
Human CYPIB1 ELISA Kit;Human ASGD6 ELISA Kit;Human CP1B ELISA Kit;Human GLC3A ELISA Kit;Human P4501B1 ELISA Kit;Human cytochrome P450 family 1 subfamily B member 1 ELISA Kit;Human cytochrome P450 1B1 ELISA Kit;Human aryl hydrocarbon hydroxylase ELISA Kit;Human cytochrome P450, family 1, subfamily B, polypeptide 1 ELISA Kit;Human cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile) ELISA Kit;Human flavoprotein-linked monooxygenase ELISA Kit;Human microsomal monooxygenase ELISA Kit;Human xenobiotic monooxygenase ELISA Kit;
Catalog
E011022
species
Human
GeneCYP1B1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome P450 1B1 (CYP1B1). No significant cross-reactivity or interference between Cytochrome P450 1B1 (CYP1B1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.59ng/mL.
Intended UseHuman CYP1B1 ELISA Kit allows for the in vitro quantitative determination of CYP1B1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1B1 (CYP1B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 1B1 (CYP1B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 1B1 (CYP1B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 1B1 (CYP1B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Cytochrome P450 1B1 Protein information
Uniprot ID CP1B1_HUMAN
Uniprot AC Q16678; Q5TZW8; Q93089; Q9H316;
UniGene Hs.154654;
GeneID 1545
KEGG hsa:1545;
Human Cytochrome P450 1B1 Protein SEQUENCE
SEQUENCE 543 AA; 60846 MW; 46B6DA7368F63EA2 CRC64;
MGTSLSPNDP WPLNPLSIQQ TTLLLLLSVL ATVHVGQRLL RQRRRQLRSA
PPGPFAWPLI GNAAAVGQAA HLSFARLARR YGDVFQIRLG SCPIVVLNGE
RAIHQALVQQ GSAFADRPAF ASFRVVSGGR SMAFGHYSEH WKVQRRAAHS
MMRNFFTRQP RSRQVLEGHV LSEARELVAL LVRGSADGAF LDPRPLTVVA
VANVMSAVCF GCRYSHDDPE FRELLSHNEE FGRTVGAGSL VDVMPWLQYF
PNPVRTVFRE FEQLNRNFSN FILDKFLRHC ESLRPGAAPR DMMDAFILSA
EKKAAGDSHG GGARLDLENV PATITDIFGA SQDTLSTALQ WLLLLFTRYP
DVQTRVQAEL DQVVGRDRLP CMGDQPNLPY VLAFLYEAMR FSSFVPVTIP
HATTANTSVL GYHIPKDTVV FVNQWSVNHD PLKWPNPENF DPARFLDKDG
LINKDLTSRV MIFSVGKRRC IGEELSKMQL FLFISILAHQ CDFRANPNEP
AKMNFSYGLT IKPKSFKVNV TLRESMELLD SAVQNLQAKE TCQ
UCSC uc032njx.2; human.;

Category

Human CYP1B1 ELISA Kit