Human CYP26B1 ELISA Kit

Alternative name
Human Cytochrome P450 26A2 ELISA Kit;Human cytochrome P450 retinoic acid-inactivating 2 ELISA Kit;Human Cytochrome P450RAI-2 ELISA Kit;Human retinoic acid-metabolizing cytochrome ELISA Kit;Human CYP26A2 ELISA Kit;Human P450RAI2 ELISA Kit;Human P450RAI-2 ELISA Kit;Human RHFCA ELISA Kit;Human cytochrome P450 family 26 subfamily B member 1 ELISA Kit;Human cytochrome P450 26B1 ELISA Kit;Human cytochrome P450 family 26 subfamily A member 1 ELISA Kit;Human cytochrome P450 retinoid metabolizing protein ELISA Kit;Human cytochrome P450, family 26, subfamily B, polypeptide 1 ELISA Kit;Human cytochrome P450, subfamily XXVIB, polypeptide 1 ELISA Kit;
Catalog
E011031
species
Human
GeneCYP26B1
SpecificityThis assay has high sensitivity and excellent specificity for detection of CYP26B1. No significant cross-reactivity or interference between CYP26B1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CYP26B1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CYP26B1 ELISA Kit allows for the in vitro quantitative determination of CYP26B1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: CYP26B1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CYP26B1 antibody and an CYP26B1-HRP conjugate. The assay sample and buffer are incubated together with CYP26B1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP26B1 concentration since CYP26B1 from samples and CYP26B1-HRP conjugate compete for the anti-CYP26B1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP26B1 from the sample, fewer sites are left to bind CYP26B1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP26B1 concentration in each sample is interpolated from this standard curve.
Human Cytochrome P450 26B1 Protein information
Uniprot ID CP26B_HUMAN
Uniprot AC Q9NP41;
UniGene Hs.91546;
GeneID 56603
KEGG hsa:56603;
Human Cytochrome P450 26B1 Protein SEQUENCE
SEQUENCE 512 AA; 57513 MW; A06D1D9944E6726F CRC64;
MLFEGLDLVS ALATLAACLV SVTLLLAVSQ QLWQLRWAAT RDKSCKLPIP
KGSMGFPLIG ETGHWLLQGS GFQSSRREKY GNVFKTHLLG RPLIRVTGAE
NVRKILMGEH HLVSTEWPRS TRMLLGPNTV SNSIGDIHRN KRKVFSKIFS
HEALESYLPK IQLVIQDTLR AWSSHPEAIN VYQEAQKLTF RMAIRVLLGF
SIPEEDLGHL FEVYQQFVDN VFSLPVDLPF SGYRRGIQAR QILQKGLEKA
IREKLQCTQG KDYLDALDLL IESSKEHGKE MTMQELKDGT LELIFAAYAT
TASASTSLIM QLLKHPTVLE KLRDELRAHG ILHSGGCPCE GTLRLDTLSG
LRYLDCVIKE VMRLFTPISG GYRTVLQTFE LDGFQIPKGW SVMYSIRDTH
DTAPVFKDVN VFDPDRFSQA RSEDKDGRFH YLPFGGGVRT CLGKHLAKLF
LKVLAVELAS TSRFELATRT FPRITLVPVL HPVDGLSVKF FGLDSNQNEI
LPETEAMLSA TV
UCSC uc002sih.3; human. [Q9NR63-1];

Category

Human CYP26B1 ELISA Kit