Human CYP2A6 ELISA Kit

Alternative name
Human 1,4-cineole 2-exo-monooxygenase ELISA Kit;Human CYPIIA6 ELISA Kit;Human coumarin 7-hydroxylase ELISA Kit;Human cytochrome P450 IIA3 ELISA Kit;Human cytochrome P450(I) ELISA Kit;Human CYP2A3 ELISA Kit;Human CPA6 ELISA Kit;Human CYP2A ELISA Kit;Human P450C2A ELISA Kit;Human P450PB ELISA Kit;Human cytochrome P450 family 2 subfamily A member 6 ELISA Kit;Human cytochrome P450 2A6 ELISA Kit;Human cytochrome P450, family 2, subfamily A, polypeptide 6 ELISA Kit;Human cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 6 ELISA Kit;Human flavoprotein-linked monooxygenase ELISA Kit;Human xenobiotic monooxygenase ELISA Kit;
Catalog
E011038
species
Human
GeneCYP2A6
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.938ng/ml
Intended UseHuman CYP2A6 ELISA Kit allows for the in vitro quantitative determination of CYP2A6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology anti-Human CYP2A6 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CYP2A6 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CYP2A6 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CYP2A6 can be calculated.
Human Cytochrome P450 2A6 Protein information
Uniprot ID CP2A6_HUMAN
Uniprot AC Q4VAU1; Q9H1Z7; Q9UCU0; Q9UK48;
UniGene Hs.250615;
GeneID 1548
KEGG hsa:1548;
Human Cytochrome P450 2A6 Protein SEQUENCE
SEQUENCE 494 AA; 56501 MW; 8C05CBF0EFC698AF CRC64;
MLASGMLLVA LLVCLTVMVL MSVWQQRKSK GKLPPGPTPL PFIGNYLQLN
TEQMYNSLMK ISERYGPVFT IHLGPRRVVV LCGHDAVREA LVDQAEEFSG
RGEQATFDWV FKGYGVVFSN GERAKQLRRF SIATLRDFGV GKRGIEERIQ
EEAGFLIDAL RGTGGANIDP TFFLSRTVSN VISSIVFGDR FDYKDKEFLS
LLRMMLGIFQ FTSTSTGQLY EMFSSVMKHL PGPQQQAFQL LQGLEDFIAK
KVEHNQRTLD PNSPRDFIDS FLIRMQEEEK NPNTEFYLKN LVMTTLNLFI
GGTETVSTTL RYGFLLLMKH PEVEAKVHEE IDRVIGKNRQ PKFEDRAKMP
YMEAVIHEIQ RFGDVIPMSL ARRVKKDTKF RDFFLPKGTE VFPMLGSVLR
DPSFFSNPQD FNPQHFLNEK GQFKKSDAFV PFSIGKRNCF GEGLARMELF
LFFTTVMQNF RLKSSQSPKD IDVSPKHVGF ATIPRNYTMS FLPR
UCSC uc002opl.4; human.;

Category

Human CYP2A6 ELISA Kit