Human CYGB ELISA Kit

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  • Alternative name

    Human histoglobin ELISA Kit;Human HGB ELISA Kit;Human stellate cell activation-associated protein ELISA Kit;Human STAP ELISA Kit;Human cytoglobin ELISA Kit;

  • Catalog
    E011139
  • species
    Human
  • GeneCYGB
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytoglobin (CYGB). No significant cross-reactivity or interference between Cytoglobin (CYGB) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.064ng/mL.
  • Intended UseHuman CYGB ELISA Kit allows for the in vitro quantitative determination of CYGB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytoglobin (CYGB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytoglobin (CYGB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytoglobin (CYGB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytoglobin (CYGB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cytoglobin Protein information
  • Uniprot ID CYGB_HUMAN
  • Uniprot AC Q8WWM9; Q541Y7; Q8N2X5;
  • UniGene Hs.95120;
  • GeneID 114757
  • KEGG hsa:114757;
  • Human Cytoglobin Protein SEQUENCE
  • SEQUENCE 190 AA; 21405 MW; B5D7F9976D0BA118 CRC64;

    MEKVPGEMEI ERRERSEELS EAERKAVQAM WARLYANCED VGVAILVRFF

    VNFPSAKQYF SQFKHMEDPL EMERSPQLRK HACRVMGALN TVVENLHDPD

    KVSSVLALVG KAHALKHKVE PVYFKILSGV ILEVVAEEFA SDFPPETQRA

    WAKLRGLIYS HVTAAYKEVG WVQQVPNATT PPATLPSSGP

  • UCSC uc002jru.3; human.;



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