Human CTLA4 ELISA Kit

Alternative name
Human Cytotoxic T-lymphocyte-associated antigen 4 ELISA Kit;Human CTLA-4 ELISA Kit;Human CD152 ELISA Kit;Human ALPS5 ELISA Kit;Human CD ELISA Kit;Human CELIAC3 ELISA Kit;Human GRD4 ELISA Kit;Human GSE ELISA Kit;Human IDDM12 ELISA Kit;Human cytotoxic T-lymphocyte associated protein 4 ELISA Kit;Human cytotoxic T-lymphocyte protein 4 ELISA Kit;Human CD152 isoform ELISA Kit;Human celiac disease 3 ELISA Kit;Human cytotoxic T lymphocyte associated antigen 4 short spliced form ELISA Kit;Human cytotoxic T-lymphocyte-associated serine esterase-4 ELISA Kit;Human insulin-dependent diabetes mellitus 12 ELISA Kit;Human ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 ELISA Kit;
Catalog
E011282
species
Human
GeneCTLA4
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4). No significant cross-reactivity or interference between Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 13.3pg/mL.
Intended UseHuman CTLA4 ELISA Kit allows for the in vitro quantitative determination of CTLA4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Cytotoxic T-lymphocyte protein 4 Protein information
Uniprot ID CTLA4_HUMAN
Uniprot AC Q8WXJ1; Q96P43; Q9UKN9;
UniGene Hs.247824;
GeneID 1493
KEGG hsa:1493;
Human Cytotoxic T-lymphocyte protein 4 Protein SEQUENCE
SEQUENCE 223 AA; 24656 MW; 6F9466FB2E139A5A CRC64;
MACLGFQRHK AQLNLATRTW PCTLLFFLLF IPVFCKAMHV AQPAVVLASS
RGIASFVCEY ASPGKATEVR VTVLRQADSQ VTEVCAATYM MGNELTFLDD
SICTGTSSGN QVNLTIQGLR AMDTGLYICK VELMYPPPYY LGIGNGTQIY
VIDPEPCPDS DFLLWILAAV SSGLFFYSFL LTAVSLSKML KKRSPLTTGV
YVKMPPTEPE CEKQFQPYFI PIN
UCSC uc002vak.3; human. [P16410-1];

Category

Human CTLA4 ELISA Kit