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Human DCN ELISA Kit

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  • Alternative name

    Human bone proteoglycan II ELISA Kit;Human PG-S2 ELISA Kit;Human PG40 ELISA Kit;Human SLRR1B ELISA Kit;Human CSCD ELISA Kit;Human DSPG2 ELISA Kit;Human PGII ELISA Kit;Human PGS2 ELISA Kit;Human decorin ELISA Kit;Human dermatan sulphate proteoglycans II ELISA Kit;Human proteoglycan core protein ELISA Kit;Human small leucine-rich protein 1B ELISA Kit;
  • Catalog
    E011454
  • species
    Human
  • GeneDCN
  • SpecificityNatural and recombinant human Decorin
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman DCN ELISA Kit allows for the in vitro quantitative determination of DCN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human Decorin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Decorin has been precoated onto 96-well plates. Standards(NSO,D31-K359) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Decorin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Decorin amount of sample captured in plate. Background: Decorin is a proteoglycan on average 90 - 140 kilodaltons (kD) in size.It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.
  • Human Decorin Protein information
  • Uniprot ID PGS2_HUMAN
  • Uniprot AC P07585; Q9P0Z0; Q9P0Z1; Q9Y5N8; Q9Y5N9;
  • UniGene Hs.156316; Hs.530910;
  • GeneID 1634
  • KEGG hsa:1634;
  • Human Decorin Protein SEQUENCE

  • SEQUENCE 359 AA; 39747 MW; FF511E871A1A52DD CRC64;

    MKATIILLLL AQVSWAGPFQ QRGLFDFMLE DEASGIGPEV PDDRDFEPSL

    GPVCPFRCQC HLRVVQCSDL GLDKVPKDLP PDTTLLDLQN NKITEIKDGD

    FKNLKNLHAL ILVNNKISKV SPGAFTPLVK LERLYLSKNQ LKELPEKMPK

    TLQELRAHEN EITKVRKVTF NGLNQMIVIE LGTNPLKSSG IENGAFQGMK

    KLSYIRIADT NITSIPQGLP PSLTELHLDG NKISRVDAAS LKGLNNLAKL

    GLSFNSISAV DNGSLANTPH LRELHLDNNK LTRVPGGLAE HKYIQVVYLH

    NNNISVVGSS DFCPPGHNTK KASYSGVSLF SNPVQYWEIQ PSTFRCVYVR

    SAIQLGNYK

  • UCSC uc001tbo.4; human. [P07585-1];



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