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Alternative name
Human Defensin, alpha 4 ELISA Kit;Human HNP-4 ELISA Kit;Human HP-4 ELISA Kit;Human DEF4 ELISA Kit;Human HP4 ELISA Kit;Human defensin alpha 4 ELISA Kit;Human neutrophil defensin 4 ELISA Kit;Human corticostatin ELISA Kit;Human defensin, alpha 4, corticostatin ELISA Kit;Human defensin, alpha 4, preproprotein ELISA Kit;
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Catalog
E011490
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species
Human
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GeneDEFA4
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Defensin Alpha 4, Corticostatin (DEFa4).
No significant cross-reactivity or interference between Defensin Alpha 4, Corticostatin (DEFa4) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 13.2pg/mL.
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Intended UseHuman DEFA4 ELISA Kit allows for the in vitro quantitative determination of DEFA4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Alpha 4, Corticostatin (DEFa4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Alpha 4, Corticostatin (DEFa4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Alpha 4, Corticostatin (DEFa4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Alpha 4, Corticostatin (DEFa4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Human Neutrophil defensin 4 Protein information
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Uniprot ID
DEF4_HUMAN
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Uniprot AC
P12838; Q6EZF8;
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UniGene
Hs.591391;
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GeneID
1669
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KEGG
hsa:1669;
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Human Neutrophil defensin 4 Protein SEQUENCE
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SEQUENCE 97 AA; 10504 MW; FE14334631EC2FD3 CRC64;
MRIIALLAAI LLVALQVRAG PLQARGDEAP GQEQRGPEDQ DISISFAWDK
SSALQVSGST
RGMVCSCRLV FCRRTELRVG NCLIGGVSFT YCCTRVD
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UCSC
uc003wqu.2; human.;
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