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Alternative name
Delta-like protein 4 ELISA KIT; Drosophila Delta homolog 4 ELISA KIT; Delta4DLL4 ELISA KIT; Delta4 ELISA KIT
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Catalog
E011603
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species
Human
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GenedLL4
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SpecificityThis assay has high sensitivity and excellent specificity for detection of dLL4. No significant cross-reactivity or interference between dLL4 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.054ng/mL.
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Intended UseHuman dLL4 ELISA Kit allows for the in vitro quantitative determination of dLL4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of dLL4 in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to dLL4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to dLL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain dLL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of dLL4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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