Human ALDH4A1 ELISA Kit

Alternative name
Human P5C dehydrogenase ELISA Kit;Human aldehyde dehydrogenase family 4 member A1 ELISA Kit;Human L-glutamate gamma-semialdehyde dehydrogenase ELISA Kit;Human ALDH4 ELISA Kit;Human P5CDh ELISA Kit;Human P5CD ELISA Kit;Human aldehyde dehydrogenase 4 family member A1 ELISA Kit;Human delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial ELISA Kit;Human mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase ELISA Kit;
Catalog
E011610
species
Human
GeneALDH4A1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman ALDH4A1 ELISA Kit allows for the in vitro quantitative determination of ALDH4A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ALDH4A1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ALDH4A1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ALDH4A1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ALDH4A1 can be calculated.
Human Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial Protein information
Uniprot ID AL4A1_HUMAN
Uniprot AC Q96IF0; Q9UDI6;
UniGene Hs.77448;
GeneID 8659
KEGG hsa:8659;
Human Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial Protein SEQUENCE
SEQUENCE 563 AA; 61719 MW; 4D964771B7DB5FFD CRC64;
MLLPAPALRR ALLSRPWTGA GLRWKHTSSL KVANEPVLAF TQGSPERDAL
QKALKDLKGR MEAIPCVVGD EEVWTSDVQY QVSPFNHGHK VAKFCYADKS
LLNKAIEAAL AARKEWDLKP IADRAQIFLK AADMLSGPRR AEILAKTMVG
QGKTVIQAEI DAAAELIDFF RFNAKYAVEL EGQQPISVPP STNSTVYRGL
EGFVAAISPF NFTAIGGNLA GAPALMGNVV LWKPSDTAML ASYAVYRILR
EAGLPPNIIQ FVPADGPLFG DTVTSSEHLC GINFTGSVPT FKHLWKQVAQ
NLDRFHTFPR LAGECGGKNF HFVHRSADVE SVVSGTLRSA FEYGGQKCSA
CSRLYVPHSL WPQIKGRLLE EHSRIKVGDP AEDFGTFFSA VIDAKSFARI
KKWLEHARSS PSLTILAGGK CDDSVGYFVE PCIVESKDPQ EPIMKEEIFG
PVLSVYVYPD DKYKETLQLV DSTTSYGLTG AVFSQDKDVV QEATKVLRNA
AGNFYINDKS TGSIVGQQPF GGARASGTND KPGGPHYILR WTSPQVIKET
HKPLGDWSYA YMQ
UCSC uc001bbb.4; human. [P30038-1];

Category

Human ALDH4A1 ELISA Kit