Human DCK ELISA Kit

Alternative name
Human dCK ELISA Kit;Human deoxycytidine kinase ELISA Kit;Human deoxynucleoside kinase ELISA Kit;
Catalog
E011677
species
Human
GeneDCK
SpecificityThis assay has high sensitivity and excellent specificity for detection of Deoxycytidine Kinase (DCK). No significant cross-reactivity or interference between Deoxycytidine Kinase (DCK) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.055ng/mL.
Intended UseHuman DCK ELISA Kit allows for the in vitro quantitative determination of DCK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deoxycytidine Kinase (DCK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deoxycytidine Kinase (DCK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deoxycytidine Kinase (DCK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deoxycytidine Kinase (DCK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Deoxycytidine kinase Protein information
Uniprot ID DCK_HUMAN
Uniprot AC P27707; B2R8V6; Q5TZY7; Q6FI11;
UniGene Hs.709;
GeneID 1633
KEGG hsa:1633;
Human Deoxycytidine kinase Protein SEQUENCE
SEQUENCE 260 AA; 30519 MW; 626B9D2D6BED8DBC CRC64;
MATPPKRSCP SFSASSEGTR IKKISIEGNI AAGKSTFVNI LKQLCEDWEV
VPEPVARWCN VQSTQDEFEE LTMSQKNGGN VLQMMYEKPE RWSFTFQTYA
CLSRIRAQLA SLNGKLKDAE KPVLFFERSV YSDRYIFASN LYESECMNET
EWTIYQDWHD WMNNQFGQSL ELDGIIYLQA TPETCLHRIY LRGRNEEQGI
PLEYLEKLHY KHESWLLHRT LKTNFDYLQE VPILTLDVNE DFKDKYESLV
EKVKEFLSTL
UCSC uc003hfx.4; human.;

Category

Human DCK ELISA Kit

Human dCK ELISA Kit;Human deoxycytidine kinase ELISA Kit;Human deoxynucleoside kinase ELISA Kit;