Human DNASE1 ELISA Kit

Alternative name
Human deoxyribonuclease I ELISA Kit;Human DNase I ELISA Kit;Human DNL1 ELISA Kit;Human DRNI ELISA Kit;Human deoxyribonuclease 1 ELISA Kit;Human deoxyribonuclease-1 ELISA Kit;Human DNase I, lysosomal ELISA Kit;Human Dornase alfa ELISA Kit;Human human urine deoxyribonuclease I ELISA Kit;
Catalog
E011697
species
Human
GeneDNASE1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Deoxyribonuclease I (DNASE1). No significant cross-reactivity or interference between Deoxyribonuclease I (DNASE1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 34pg/mL.
Intended UseHuman DNASE1 ELISA Kit allows for the in vitro quantitative determination of DNASE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deoxyribonuclease I (DNASE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deoxyribonuclease I (DNASE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deoxyribonuclease I (DNASE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deoxyribonuclease I (DNASE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Deoxyribonuclease-1 Protein information
Uniprot ID DNAS1_HUMAN
Uniprot AC P24855; B4DV35; Q14UU9; Q14UV0;
UniGene Hs.629638; Hs.733045;
GeneID 1773
KEGG hsa:1773;
Human Deoxyribonuclease-1 Protein SEQUENCE
SEQUENCE 282 AA; 31434 MW; 040042E2D23555B6 CRC64;
MRGMKLLGAL LALAALLQGA VSLKIAAFNI QTFGETKMSN ATLVSYIVQI
LSRYDIALVQ EVRDSHLTAV GKLLDNLNQD APDTYHYVVS EPLGRNSYKE
RYLFVYRPDQ VSAVDSYYYD DGCEPCGNDT FNREPAIVRF FSRFTEVREF
AIVPLHAAPG DAVAEIDALY DVYLDVQEKW GLEDVMLMGD FNAGCSYVRP
SQWSSIRLWT SPTFQWLIPD SADTTATPTH CAYDRIVVAG MLLRGAVVPD
SALPFNFQAA YGLSDQLAQA ISDHYPVEVM LK
UCSC uc002cvr.4; human. [P24855-1];

Category

Human DNASE1 ELISA Kit

Human deoxyribonuclease I ELISA Kit;Human DNase I ELISA Kit;Human DNL1 ELISA Kit;Human DRNI ELISA Kit;Human deoxyribonuclease 1 ELISA Kit;Human deoxyribonuclease-1 ELISA Kit;Human DNase I, lysosomal ELISA Kit;Human Dornase alfa ELISA Kit;Human human urine deoxyribonuclease I ELISA Kit;