Human DUT ELISA Kit

Alternative name
Human dUTPase ELISA Kit;Human dUTP pyrophosphatase ELISA Kit;Human deoxyuridine triphosphatase ELISA Kit;Human deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial ELISA Kit;Human dUTP diphosphatase ELISA Kit;Human dUTP nucleotidohydrolase ELISA Kit;
Catalog
E011713
species
Human
GeneDUT
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman DUT ELISA Kit allows for the in vitro quantitative determination of DUT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human DUT antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human DUT antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human DUT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human DUT can be calculated.
Human Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial Protein information
Uniprot ID DUT_HUMAN
Uniprot AC Q96Q81;
UniGene Hs.527980;
GeneID 1854
KEGG hsa:1854;
Human Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial Protein SEQUENCE
SEQUENCE 252 AA; 26563 MW; 8E2EBC9ED5B0FAD2 CRC64;
MTPLCPRPAL CYHFLTSLLR SAMQNARGAR QRAEAAVLSG PGPPLGRAAQ
HGIPRPLSSA GRLSQGCRGA STVGAAGWKG ELPKAGGSPA PGPETPAISP
SKRARPAEVG GMQLRFARLS EHATAPTRGS ARAAGYDLYS AYDYTIPPME
KAVVKTDIQI ALPSGCYGRV APRSGLAAKH FIDVGAGVID EDYRGNVGVV
LFNFGKEKFE VKKGDRIAQL ICERIFYPEI EEVQALDDTE RGSGGFGSTG
KN
UCSC uc001zws.4; human. [P33316-3];

Category

Human DUT ELISA Kit

Human dUTPase ELISA Kit;Human dUTP pyrophosphatase ELISA Kit;Human deoxyuridine triphosphatase ELISA Kit;Human deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial ELISA Kit;Human dUTP diphosphatase ELISA Kit;Human dUTP nucleotidohydrolase ELISA Kit;