Human DES ELISA Kit

Alternative name
Human CSM1 ELISA Kit;Human CSM2 ELISA Kit;Human LGMD2R ELISA Kit;Human desmin ELISA Kit;Human intermediate filament protein ELISA Kit;Human mutant desmin p.K241E ELISA Kit;
Catalog
E011763
species
Human
GeneDES
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Intended Uses For detection of Desmin in human serum, plasma, urine, tissue samples or cell
Intended UseHuman DES ELISA Kit allows for the in vitro quantitative determination of DES , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular; Brain Disorders & Neuroscience; Research Assays; Assay Categories
Product Description
specificalPrinciple of the assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-Desmin antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-Desmin antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Desmin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Desmin can be calculated. Background: Desmin is a type III intermediate filament found near the Z line in sarcomeres. It was first described in 1976, first purified in 1977, the gene was cloned in 1989, and the first knock-out mouse was created in 1996. It is one of the earliest myogenic markers, both in heart and somites, and is expressed in satellite stem cells and replicating myoblasts. The desmin provides attachments between the terminal Z disc and membrane-associated proteins to form a forcetransmitting system that parallels the thin filaments at myotendinous junctions. Desmin is also important in muscle cell architecture and structure since it connects many components of the cytoplasm. Finally, desmin may be important in mitochondria function. When desmin is not functioning properly there is improper mitochondrial distribution, number, morphology and function.
Human Desmin Protein information
Uniprot ID DESM_HUMAN
Uniprot AC Q8NEU6; Q8TAC4; Q8TCX2; Q8TD99; Q9UHN5; Q9UJ80;
UniGene Hs.594952;
GeneID 1674
KEGG hsa:1674;
Human Desmin Protein SEQUENCE
SEQUENCE 470 AA; 53536 MW; 1B5D9EA93C3BB319 CRC64;
MSQAYSSSQR VSSYRRTFGG APGFPLGSPL SSPVFPRAGF GSKGSSSSVT
SRVYQVSRTS GGAGGLGSLR ASRLGTTRTP SSYGAGELLD FSLADAVNQE
FLTTRTNEKV ELQELNDRFA NYIEKVRFLE QQNAALAAEV NRLKGREPTR
VAELYEEELR ELRRQVEVLT NQRARVDVER DNLLDDLQRL KAKLQEEIQL
KEEAENNLAA FRADVDAATL ARIDLERRIE SLNEEIAFLK KVHEEEIREL
QAQLQEQQVQ VEMDMSKPDL TAALRDIRAQ YETIAAKNIS EAEEWYKSKV
SDLTQAANKN NDALRQAKQE MMEYRHQIQS YTCEIDALKG TNDSLMRQMR
ELEDRFASEA SGYQDNIARL EEEIRHLKDE MARHLREYQD LLNVKMALDV
EIATYRKLLE GEESRINLPI QTYSALNFRE TSPEQRGSEV HTKKTVMIKT
IETRDGEVVS EATQQQHEVL

Category

Human DES ELISA Kit

Human CSM1 ELISA Kit;Human CSM2 ELISA Kit;Human LGMD2R ELISA Kit;Human desmin ELISA Kit;Human intermediate filament protein ELISA Kit;Human mutant desmin p.K241E ELISA Kit;