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Human QDPR ELISA Kit

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  • Alternative name

    Human HDHPR ELISA Kit;Human quinoid dihydropteridine reductase ELISA Kit;Human short chain dehydrogenase/reductase family 33C member 1 ELISA Kit;Human DHPR ELISA Kit;Human SDR33C1 ELISA Kit;Human PKU2 ELISA Kit;Human dihydropteridine reductase ELISA Kit;Human 6,7-dihydropteridine reductase ELISA Kit;Human testis secretory sperm-binding protein Li 236P ELISA Kit;
  • Catalog
    E011939
  • species
    Human
  • GeneQDPR
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of QDPR. No significant cross-reactivity or interference between QDPR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between QDPR and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman QDPR ELISA Kit allows for the in vitro quantitative determination of QDPR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: QDPR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-QDPR antibody and an QDPR-HRP conjugate. The assay sample and buffer are incubated together with QDPR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the QDPR concentration since QDPR from samples and QDPR-HRP conjugate compete for the anti-QDPR antibody binding site. Since the number of sites is limited, as more sites are occupied by QDPR from the sample, fewer sites are left to bind QDPR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The QDPR concentration in each sample is interpolated from this standard curve.
  • Human Dihydropteridine reductase Protein information
  • Uniprot ID DHPR_HUMAN
  • Uniprot AC P09417; A8K158; B3KW71; Q53F52; Q9H3M5;
  • UniGene Hs.75438;
  • GeneID 5860
  • KEGG hsa:5860;
  • Human Dihydropteridine reductase Protein SEQUENCE

  • SEQUENCE 244 AA; 25790 MW; 0852F9F0CA38AB1C CRC64;

    MAAAAAAGEA RRVLVYGGRG ALGSRCVQAF RARNWWVASV DVVENEEASA

    SIIVKMTDSF TEQADQVTAE VGKLLGEEKV DAILCVAGGW AGGNAKSKSL

    FKNCDLMWKQ SIWTSTISSH LATKHLKEGG LLTLAGAKAA LDGTPGMIGY

    GMAKGAVHQL CQSLAGKNSG MPPGAAAIAV LPVTLDTPMN RKSMPEADFS

    SWTPLEFLVE TFHDWITGKN RPSSGSLIQV VTTEGRTELT PAYF

  • UCSC uc003gpd.4; human. [P09417-1];



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