Human DPYS ELISA Kit

Alternative name
Human DHP ELISA Kit;Human DHPase ELISA Kit;Human dihydropyrimidine amidohydrolase ELISA Kit;Human hydantoinase ELISA Kit;Human dihydropyrimidinase ELISA Kit;
Catalog
E011941
species
Human
GeneDPYS
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dihydropyrimidinase (DPYS). No significant cross-reactivity or interference between Dihydropyrimidinase (DPYS) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.31ng/mL.
Intended UseHuman DPYS ELISA Kit allows for the in vitro quantitative determination of DPYS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydropyrimidinase (DPYS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydropyrimidinase (DPYS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydropyrimidinase (DPYS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydropyrimidinase (DPYS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Dihydropyrimidinase Protein information
Uniprot ID DPYS_HUMAN
Uniprot AC Q14117;
UniGene Hs.443161;
GeneID 1807
KEGG hsa:1807;
Human Dihydropyrimidinase Protein SEQUENCE
SEQUENCE 519 AA; 56630 MW; 882E33D7C49D6ECC CRC64;
MAAPSRLLIR GGRVVNDDFS EVADVLVEDG VVRALGHDLL PPGGAPAGLR
VLDAAGKLVL PGGIDTHTHM QFPFMGSRSI DDFHQGTKAA LSGGTTMIID
FAIPQKGGSL IEAFETWRSW ADPKVCCDYS LHVAVTWWSD QVKEEMKILV
QDKGVNSFKM FMAYKDLYMV TDLELYEAFS RCKEIGAIAQ VHAENGDLIA
EGAKKMLALG ITGPEGHELC RPEAVEAEAT LRAITIASAV NCPLYIVHVM
SKSAAKVIAD ARRDGKVVYG EPIAASLGTD GTHYWNKEWH HAAHHVMGPP
LRPDPSTPDF LMNLLANDDL TTTGTDNCTF NTCQKALGKD DFTKIPNGVN
GVEDRMSVIW EKGVHSGKMD ENRFVAVTST NAAKIFNLYP RKGRIAVGSD
ADIVIWDPKG TRTISAKTHH QAVNFNIFEG MVCHGVPLVT ISRGKVVYEA
GVFSVTAGDG KFIPRKPFAE YIYKRIKQRD RTCTPTPVER APYKGEVATL
KSRVTKEDAT AGTRKQAHP
UCSC uc003yly.5; human.;

Category

Human DPYS ELISA Kit

Human DHP ELISA Kit;Human DHPase ELISA Kit;Human dihydropyrimidine amidohydrolase ELISA Kit;Human hydantoinase ELISA Kit;Human dihydropyrimidinase ELISA Kit;