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Human DDAH2 ELISA Kit

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  • Alternative name

    Human DDAH-2 ELISA Kit;Human dimethylarginine dimethylaminohydrolase 2 ELISA Kit;Human DDAHII ELISA Kit;Human dimethylargininase-2 ELISA Kit;Human Protein G6a ELISA Kit;Human S-phase protein ELISA Kit;Human DDAH ELISA Kit;Human G6a ELISA Kit;Human NG30 ELISA Kit;Human HEL-S-277 ELISA Kit;Human N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 ELISA Kit;Human dimethylarginine dimethylaminohydrolase II ELISA Kit;Human epididymis secretory protein Li 277 ELISA Kit;Human testis tissue sperm-binding protein Li 54e ELISA Kit;
  • Catalog
    E011976
  • species
    Human
  • GeneDDAH2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Dimethylarginine Dimethylaminohydrolase 2 (DDAH2). No significant cross-reactivity or interference between Dimethylarginine Dimethylaminohydrolase 2 (DDAH2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.116ng/mL.
  • Intended UseHuman DDAH2 ELISA Kit allows for the in vitro quantitative determination of DDAH2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dimethylarginine Dimethylaminohydrolase 2 (DDAH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dimethylarginine Dimethylaminohydrolase 2 (DDAH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dimethylarginine Dimethylaminohydrolase 2 (DDAH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dimethylarginine Dimethylaminohydrolase 2 (DDAH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 Protein information
  • Uniprot ID DDAH2_HUMAN
  • Uniprot AC O95865; A2BEZ7;
  • UniGene Hs.247362;
  • GeneID 23564
  • KEGG hsa:23564;
  • Human N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 Protein SEQUENCE

  • SEQUENCE 285 AA; 29644 MW; B3D9B00F29492548 CRC64;

    MGTPGEGLGR CSHALIRGVP ESLASGEGAG AGLPALDLAK AQREHGVLGG

    KLRQRLGLQL LELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV

    DGVRKALQDL GLRIVEIGDE NATLDGTDVL FTGREFFVGL SKWTNHRGAE

    IVADTFRDFA VSTVPVSGPS HLRGLCGMGG PRTVVAGSSD AAQKAVRAMA

    VLTDHPYASL TLPDDAAADC LFLRPGLPGV PPFLLHRGGG DLPNSQEALQ

    KLSDVTLVPV SCSELEKAGA GLSSLCLVLS TRPHS




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