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Human DKK3 ELISA Kit

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  • Alternative name

    Human dickkopf-3 ELISA Kit;Human dkk-3 ELISA Kit;Human hDkk-3 ELISA Kit;Human REIC ELISA Kit;Human RIG ELISA Kit;Human dickkopf WNT signaling pathway inhibitor 3 ELISA Kit;Human dickkopf-related protein 3 ELISA Kit;Human RIG-like 5-6 ELISA Kit;Human RIG-like 7-1 ELISA Kit;Human dickkopf 3 homolog ELISA Kit;Human dickkopf homolog 3 ELISA Kit;Human regulated in glioma ELISA Kit;
  • Catalog
    E012093
  • species
    Human
  • GeneDKK3
  • SpecificityNatural and recombinant human DKK-3
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman DKK3 ELISA Kit allows for the in vitro quantitative determination of DKK3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human DKK-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DKK-3 has been precoated onto 96-well plates. Standards(NSO, A22-I350) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DKK-3 amount of sample captured in plate. Background: Dickkopf-related protein 3 is a protein that in humans is encoded by the DKK3 gene. This gene encodes a protein that is a member of the dickkopf family. It is mapped to 11p15.3. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues, and are secreted when expressed in 293T cells. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Human Dickkopf-related protein 3 Protein information
  • Uniprot ID DKK3_HUMAN
  • Uniprot AC Q9UBP4; A8K1I2; D3DQW1; Q9ULB7;
  • UniGene Hs.292156; Hs.731954;
  • GeneID 27122
  • KEGG hsa:27122;
  • Human Dickkopf-related protein 3 Protein SEQUENCE

  • SEQUENCE 350 AA; 38390 MW; 734504122B40AFEE CRC64;

    MQRLGATLLC LLLAAAVPTA PAPAPTATSA PVKPGPALSY PQEEATLNEM

    FREVEELMED TQHKLRSAVE EMEAEEAAAK ASSEVNLANL PPSYHNETNT

    DTKVGNNTIH VHREIHKITN NQTGQMVFSE TVITSVGDEE GRRSHECIID

    EDCGPSMYCQ FASFQYTCQP CRGQRMLCTR DSECCGDQLC VWGHCTKMAT

    RGSNGTICDN QRDCQPGLCC AFQRGLLFPV CTPLPVEGEL CHDPASRLLD

    LITWELEPDG ALDRCPCASG LLCQPHSHSL VYVCKPTFVG SRDQDGEILL

    PREVPDEYEV GSFMEEVRQE LEDLERSLTE EMALREPAAA AAALLGGEEI

  • UCSC uc001mjv.4; human.;



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