Human DLD ELISA Kit

Alternative name
Human dihydrolipoamide dehydrogenase ELISA Kit;Human glycine cleavage system L protein ELISA Kit;Human GCSL ELISA Kit;Human LAD ELISA Kit;Human PHE3 ELISA Kit;Human DLDD ELISA Kit;Human DLDH ELISA Kit;Human E3 ELISA Kit;Human dihydrolipoyl dehydrogenase, mitochondrial ELISA Kit;Human E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex ELISA Kit;Human diaphorase ELISA Kit;Human glycine cleavage system protein L ELISA Kit;Human lipoamide dehydrogenase ELISA Kit;Human lipoamide reductase ELISA Kit;Human lipoyl dehydrogenase ELISA Kit;
Catalog
E012101
species
Human
GeneDLD
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human DLD. No significant cross-reactivity or interference between Human DLD and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human DLD and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.938mU/mL
Intended UseHuman DLD ELISA Kit allows for the in vitro quantitative determination of DLD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DLD in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DLD. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human DLD and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DLD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human DLD. You can calculate the concentration of Human DLD in the samples by comparing the OD of the samples to the standard curve.
Human Dihydrolipoyl dehydrogenase, mitochondrial Protein information
Uniprot ID DLDH_HUMAN
Uniprot AC P09622; B2R5X0; B4DHG0; B4DT69; Q14131; Q14167; Q59EV8; Q8WTS4;
UniGene Hs.131711;
GeneID 1738
KEGG hsa:1738;
Human Dihydrolipoyl dehydrogenase, mitochondrial Protein SEQUENCE
SEQUENCE 509 AA; 54177 MW; 7613492C516F3835 CRC64;
MQSWSRVYCS LAKRGHFNRI SHGLQGLSAV PLRTYADQPI DADVTVIGSG
PGGYVAAIKA AQLGFKTVCI EKNETLGGTC LNVGCIPSKA LLNNSHYYHM
AHGKDFASRG IEMSEVRLNL DKMMEQKSTA VKALTGGIAH LFKQNKVVHV
NGYGKITGKN QVTATKADGG TQVIDTKNIL IATGSEVTPF PGITIDEDTI
VSSTGALSLK KVPEKMVVIG AGVIGVELGS VWQRLGADVT AVEFLGHVGG
VGIDMEISKN FQRILQKQGF KFKLNTKVTG ATKKSDGKID VSIEAASGGK
AEVITCDVLL VCIGRRPFTK NLGLEELGIE LDPRGRIPVN TRFQTKIPNI
YAIGDVVAGP MLAHKAEDEG IICVEGMAGG AVHIDYNCVP SVIYTHPEVA
WVGKSEEQLK EEGIEYKVGK FPFAANSRAK TNADTDGMVK ILGQKSTDRV
LGAHILGPGA GEMVNEAALA LEYGASCEDI ARVCHAHPTL SEAFREANLA
ASFGKSINF
UCSC uc003vet.5; human. [P09622-1];

Category

Human DLD ELISA Kit