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Human DNMT3A ELISA Kit

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  • Alternative name

    Human Dnmt3a ELISA Kit;Human DNA methyltransferase HsaIIIA ELISA Kit;Human DNA MTase HsaIIIA ELISA Kit;Human M.HsaIIIA ELISA Kit;Human DNMT3A2 ELISA Kit;Human TBRS ELISA Kit;Human DNA methyltransferase 3 alpha ELISA Kit;Human DNA (cytosine-5)-methyltransferase 3A ELISA Kit;Human DNA (cytosine-5-)-methyltransferase 3 alpha ELISA Kit;Human DNA cytosine methyltransferase 3A2 ELISA Kit;
  • Catalog
    E012145
  • species
    Human
  • GeneDNMT3A
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DNA Methyltransferase 3A (DNMT3A). No significant cross-reactivity or interference between DNA Methyltransferase 3A (DNMT3A) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.061ng/mL.
  • Intended UseHuman DNMT3A ELISA Kit allows for the in vitro quantitative determination of DNMT3A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Methyltransferase 3A (DNMT3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Methyltransferase 3A (DNMT3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Methyltransferase 3A (DNMT3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNA Methyltransferase 3A (DNMT3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human DNA (cytosine-5)-methyltransferase 3A Protein information
  • Uniprot ID DNM3A_HUMAN
  • Uniprot AC Q9Y6K1; E9PEB8; Q86TE8; Q86XF5; Q8IZV0; Q8WXU9;
  • UniGene Hs.515840;
  • GeneID 1788
  • KEGG hsa:1788;
  • Human DNA (cytosine-5)-methyltransferase 3A Protein SEQUENCE

  • SEQUENCE 912 AA; 101858 MW; BD1FF7C5B4F54A33 CRC64;

    MPAMPSSGPG DTSSSAAERE EDRKDGEEQE EPRGKEERQE PSTTARKVGR

    PGRKRKHPPV ESGDTPKDPA VISKSPSMAQ DSGASELLPN GDLEKRSEPQ

    PEEGSPAGGQ KGGAPAEGEG AAETLPEASR AVENGCCTPK EGRGAPAEAG

    KEQKETNIES MKMEGSRGRL RGGLGWESSL RQRPMPRLTF QAGDPYYISK

    RKRDEWLARW KREAEKKAKV IAGMNAVEEN QGPGESQKVE EASPPAVQQP

    TDPASPTVAT TPEPVGSDAG DKNATKAGDD EPEYEDGRGF GIGELVWGKL

    RGFSWWPGRI VSWWMTGRSR AAEGTRWVMW FGDGKFSVVC VEKLMPLSSF

    CSAFHQATYN KQPMYRKAIY EVLQVASSRA GKLFPVCHDS DESDTAKAVE

    VQNKPMIEWA LGGFQPSGPK GLEPPEEEKN PYKEVYTDMW VEPEAAAYAP

    PPPAKKPRKS TAEKPKVKEI IDERTRERLV YEVRQKCRNI EDICISCGSL

    NVTLEHPLFV GGMCQNCKNC FLECAYQYDD DGYQSYCTIC CGGREVLMCG

    NNNCCRCFCV ECVDLLVGPG AAQAAIKEDP WNCYMCGHKG TYGLLRRRED

    WPSRLQMFFA NNHDQEFDPP KVYPPVPAEK RKPIRVLSLF DGIATGLLVL

    KDLGIQVDRY IASEVCEDSI TVGMVRHQGK IMYVGDVRSV TQKHIQEWGP

    FDLVIGGSPC NDLSIVNPAR KGLYEGTGRL FFEFYRLLHD ARPKEGDDRP

    FFWLFENVVA MGVSDKRDIS RFLESNPVMI DAKEVSAAHR ARYFWGNLPG

    MNRPLASTVN DKLELQECLE HGRIAKFSKV RTITTRSNSI KQGKDQHFPV

    FMNEKEDILW CTEMERVFGF PVHYTDVSNM SRLARQRLLG RSWSVPVIRH

    LFAPLKEYFA CV

  • UCSC uc002rgb.5; human. [Q9Y6K1-1];



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