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Human DNTT ELISA Kit

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  • Alternative name

    Human terminal addition enzyme ELISA Kit;Human terminal deoxynucleotidyltransferase ELISA Kit;Human terminal transferase ELISA Kit;Human TDT ELISA Kit;Human DNA nucleotidylexotransferase ELISA Kit;Human nucleosidetriphosphate:DNA deoxynucleotidylexotransferase ELISA Kit;Human terminal deoxyribonucleotidyltransferase ELISA Kit;
  • Catalog
    E012154
  • species
    Human
  • GeneDNTT
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman DNTT ELISA Kit allows for the in vitro quantitative determination of DNTT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human DNTT antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human DNTT antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human DNTT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human DNTT can be calculated.
  • Human DNA nucleotidylexotransferase Protein information
  • Uniprot ID TDT_HUMAN
  • Uniprot AC P04053; Q53FH1; Q5W103; Q96E50;
  • UniGene Hs.534206;
  • GeneID 1791
  • KEGG hsa:1791;
  • Human DNA nucleotidylexotransferase Protein SEQUENCE

  • SEQUENCE 509 AA; 58536 MW; 0EC01E380FA7E5A2 CRC64;

    MDPPRASHLS PRKKRPRQTG ALMASSPQDI KFQDLVVFIL EKKMGTTRRA

    FLMELARRKG FRVENELSDS VTHIVAENNS GSDVLEWLQA QKVQVSSQPE

    LLDVSWLIEC IRAGKPVEMT GKHQLVVRRD YSDSTNPGPP KTPPIAVQKI

    SQYACQRRTT LNNCNQIFTD AFDILAENCE FRENEDSCVT FMRAASVLKS

    LPFTIISMKD TEGIPCLGSK VKGIIEEIIE DGESSEVKAV LNDERYQSFK

    LFTSVFGVGL KTSEKWFRMG FRTLSKVRSD KSLKFTRMQK AGFLYYEDLV

    SCVTRAEAEA VSVLVKEAVW AFLPDAFVTM TGGFRRGKKM GHDVDFLITS

    PGSTEDEEQL LQKVMNLWEK KGLLLYYDLV ESTFEKLRLP SRKVDALDHF

    QKCFLIFKLP RQRVDSDQSS WQEGKTWKAI RVDLVLCPYE RRAFALLGWT

    GSRQFERDLR RYATHERKMI LDNHALYDKT KRIFLKAESE EEIFAHLGLD

    YIEPWERNA

  • UCSC uc001kmf.4; human. [P04053-1];



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