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Human DDIT4 ELISA Kit

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  • Alternative name

    Human HIF-1 responsive protein RTP801 ELISA Kit;Human protein regulated in development and DNA damage response 1 ELISA Kit;Human REDD-1 ELISA Kit;Human REDD1 ELISA Kit;Human RTP801 ELISA Kit;Human Dig2 ELISA Kit;Human DNA damage inducible transcript 4 ELISA Kit;Human DNA damage-inducible transcript 4 protein ELISA Kit;
  • Catalog
    E012181
  • species
    Human
  • GeneDDIT4
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human DDIT4. No significant cross-reactivity or interference between human DDIT4 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human DDIT4 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
  • Intended UseHuman DDIT4 ELISA Kit allows for the in vitro quantitative determination of DDIT4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DDIT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DDIT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DDIT4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDIT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human DNA damage-inducible transcript 4 protein Protein information
  • Uniprot ID DDIT4_HUMAN
  • Uniprot AC Q9NX09; Q9H0S3;
  • UniGene Hs.744875;
  • GeneID 54541
  • KEGG hsa:54541;
  • Human DNA damage-inducible transcript 4 protein Protein SEQUENCE

  • SEQUENCE 232 AA; 25371 MW; 774E941EBDD08198 CRC64;

    MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE

    SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL

    QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC

    VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF

    LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC

  • UCSC uc001jsx.2; human.;



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