Human DPAGT1 ELISA Kit

Alternative name
Human GlcNAc-1-P transferase ELISA Kit;Human G1PT ELISA Kit;Human GPT ELISA Kit;Human N-acetylglucosamine-1-phosphate transferase ELISA Kit;Human DPAGT2 ELISA Kit;Human ALG7 ELISA Kit;Human CDG-Ij ELISA Kit;Human CDG1J ELISA Kit;Human CMS13 ELISA Kit;Human CMSTA2 ELISA Kit;Human D11S366 ELISA Kit;Human DGPT ELISA Kit;Human DPAGT ELISA Kit;Human UAGT ELISA Kit;Human UGAT ELISA Kit;Human dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 ELISA Kit;Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase ELISA Kit;Human GlcNAc-1-P transferase 1 ELISA Kit;Human UDP-GlcNAc:dolichyl-phosphate N-acetylglucosaminephosphotransferase ELISA Kit;Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 ELISA Kit;Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P tra ELISA Kit;Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) ELISA Kit;Human dolichyl-phosphate alpha-N-acetylglucosaminyltransferase ELISA Kit;
Catalog
E012270
species
Human
GeneDPAGT1
SpecificityThis assay has high sensitivity and excellent specificity for detection of DPAGT1. No significant cross-reactivity or interference between DPAGT1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of DPAGT1 is typically less than 0.059ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding t
Intended UseHuman DPAGT1 ELISA Kit allows for the in vitro quantitative determination of DPAGT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DPAGT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DPAGT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DPAGT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DPAGT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase Protein information
Uniprot ID GPT_HUMAN
Uniprot AC Q9H3H5; O15216; Q86WV9; Q9BWE6;
UniGene Hs.524081;
GeneID 1798
KEGG hsa:1798;
Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase Protein SEQUENCE
SEQUENCE 408 AA; 46090 MW; 0AE10EFE55E7B9E0 CRC64;
MWAFSELPMP LLINLIVSLL GFVATVTLIP AFRGHFIAAR LCGQDLNKTS
RQQIPESQGV ISGAVFLIIL FCFIPFPFLN CFVKEQCKAF PHHEFVALIG
ALLAICCMIF LGFADDVLNL RWRHKLLLPT AASLPLLMVY FTNFGNTTIV
VPKPFRPILG LHLDLGILYY VYMGLLAVFC TNAINILAGI NGLEAGQSLV
ISASIIVFNL VELEGDCRDD HVFSLYFMIP FFFTTLGLLY HNWYPSRVFV
GDTFCYFAGM TFAVVGILGH FSKTMLLFFM PQVFNFLYSL PQLLHIIPCP
RHRIPRLNIK TGKLEMSYSK FKTKSLSFLG TFILKVAESL QLVTVHQSET
EDGEFTECNN MTLINLLLKV LGPIHERNLT LLLLLLQILG SAITFSIRYQ
LVRLFYDV
UCSC uc001pvi.4; human. [Q9H3H5-1];

Category

Human DPAGT1 ELISA Kit