Human DRD2 ELISA Kit

Alternative name
Human dopamine D2 receptor ELISA Kit;Human D2DR ELISA Kit;Human D2R ELISA Kit;Human dopamine receptor D2 ELISA Kit;Human D(2) dopamine receptor ELISA Kit;Human dopamine receptor D2 isoform ELISA Kit;Human seven transmembrane helix receptor ELISA Kit;
Catalog
E012313
species
Human
GeneDRD2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman DRD2 ELISA Kit allows for the in vitro quantitative determination of DRD2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This DRD2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DRD2. This ELISA kit for researchuse only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY DRD2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DRD2 antibody and an DRD2-HRP conjugate. The assay sample and buffer are incubated together with DRD2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DRD2 concentration since DRD2 from samples and DRD2-HRP conjugate compete for the anti-DRD2 antibody binding site. Since the number of sites is limited, as more sites are occupied by DRD2 from the sample, fewer sites are left to bind DRD2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DRD2 concentration in each sample is interpolated from this standard curve.
Human D(2) dopamine receptor Protein information
Uniprot ID DRD2_HUMAN
Uniprot AC P14416; Q9NZR3; Q9UPA9;
UniGene Hs.73893;
GeneID 1813
KEGG hsa:1813;
Human D(2) dopamine receptor Protein SEQUENCE
SEQUENCE 443 AA; 50619 MW; 9BF8EA36C988A2E2 CRC64;
MDPLNLSWYD DDLERQNWSR PFNGSDGKAD RPHYNYYATL LTLLIAVIVF
GNVLVCMAVS REKALQTTTN YLIVSLAVAD LLVATLVMPW VVYLEVVGEW
KFSRIHCDIF VTLDVMMCTA SILNLCAISI DRYTAVAMPM LYNTRYSSKR
RVTVMISIVW VLSFTISCPL LFGLNNADQN ECIIANPAFV VYSSIVSFYV
PFIVTLLVYI KIYIVLRRRR KRVNTKRSSR AFRAHLRAPL KGNCTHPEDM
KLCTVIMKSN GSFPVNRRRV EAARRAQELE MEMLSSTSPP ERTRYSPIPP
SHHQLTLPDP SHHGLHSTPD SPAKPEKNGH AKDHPKIAKI FEIQTMPNGK
TRTSLKTMSR RKLSQQKEKK ATQMLAIVLG VFIICWLPFF ITHILNIHCD
CNIPPVLYSA FTWLGYVNSA VNPIIYTTFN IEFRKAFLKI LHC
UCSC uc001pnz.4; human. [P14416-1];

Category

Human DRD2 ELISA Kit

Human dopamine D2 receptor ELISA Kit;Human D2DR ELISA Kit;Human D2R ELISA Kit;Human dopamine receptor D2 ELISA Kit;Human D(2) dopamine receptor ELISA Kit;Human dopamine receptor D2 isoform ELISA Kit;Human seven transmembrane helix receptor ELISA Kit;