Human DRD3 ELISA Kit

Alternative name
Human Dopamine D3 receptor ELISA Kit;Human D3DR ELISA Kit;Human ETM1 ELISA Kit;Human FET1 ELISA Kit;Human dopamine receptor D3 ELISA Kit;Human D(3) dopamine receptor ELISA Kit;Human essential tremor 1 ELISA Kit;
Catalog
E012316
species
Human
GeneDRD3
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman DRD3 ELISA Kit allows for the in vitro quantitative determination of DRD3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalIntended Uses: This DRD3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DRD3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: DRD3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DRD3 antibody and an DRD3-HRP conjugate. The assay sample and buffer are incubated together with DRD3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DRD3 concentration since DRD3 from samples and DRD3-HRP conjugate compete for the anti-DRD3 antibody binding site. Since the number of sites is limited, as more sites are occupied by DRD3 from the sample, fewer sites are left to bind DRD3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DRD3 concentration in each sample is interpolated from this standard curve.
Human D(3) dopamine receptor Protein information
Uniprot ID DRD3_HUMAN
Uniprot AC P35462; A1A4V5; Q4VBM8;
UniGene Hs.121478;
GeneID 1814
KEGG hsa:1814;
Human D(3) dopamine receptor Protein SEQUENCE
SEQUENCE 400 AA; 44225 MW; 2CDA789D78378DDA CRC64;
MASLSQLSSH LNYTCGAENS TGASQARPHA YYALSYCALI LAIVFGNGLV
CMAVLKERAL QTTTNYLVVS LAVADLLVAT LVMPWVVYLE VTGGVWNFSR
ICCDVFVTLD VMMCTASILN LCAISIDRYT AVVMPVHYQH GTGQSSCRRV
ALMITAVWVL AFAVSCPLLF GFNTTGDPTV CSISNPDFVI YSSVVSFYLP
FGVTVLVYAR IYVVLKQRRR KRILTRQNSQ CNSVRPGFPQ QTLSPDPAHL
ELKRYYSICQ DTALGGPGFQ ERGGELKREE KTRNSLSPTI APKLSLEVRK
LSNGRLSTSL KLGPLQPRGV PLREKKATQM VAIVLGAFIV CWLPFFLTHV
LNTHCQTCHV SPELYSATTW LGYVNSALNP VIYTTFNIEF RKAFLKILSC

Category

Human DRD3 ELISA Kit

Human Dopamine D3 receptor ELISA Kit;Human D3DR ELISA Kit;Human ETM1 ELISA Kit;Human FET1 ELISA Kit;Human dopamine receptor D3 ELISA Kit;Human D(3) dopamine receptor ELISA Kit;Human essential tremor 1 ELISA Kit;