Human DTX1 ELISA Kit

Alternative name
Human protein deltex-1 ELISA Kit;Human deltex1 ELISA Kit;Human hDTX1 ELISA Kit;Human RING-type E3 ubiquitin transferase DTX1 ELISA Kit;Human hDx-1 ELISA Kit;Human deltex E3 ubiquitin ligase 1 ELISA Kit;Human E3 ubiquitin-protein ligase DTX1 ELISA Kit;Human RING-type E3 ubiquitin transferase DTX1 ELISA Kit;Human deltex 1, E3 ubiquitin ligase ELISA Kit;Human deltex homolog 1 ELISA Kit;
Catalog
E012412
species
Human
GeneDTX1
SpecificityThis assay has high sensitivity and excellent specificity for detection of DTX1. No significant cross-reactivity or interference between DTX1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DTX1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman DTX1 ELISA Kit allows for the in vitro quantitative determination of DTX1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the assay: DTX1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DTX1 antibody and an DTX1-HRP conjugate. The assay sample and buffer are incubated together with DTX1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DTX1 concentration since DTX1 from samples and DTX1-HRP conjugate compete for the anti-DTX1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DTX1 from the sample, fewer sites are left to bind DTX1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DTX1 concentration in each sample is interpolated from this standard curve.
Human E3 ubiquitin-protein ligase DTX1 Protein information
Uniprot ID DTX1_HUMAN
Uniprot AC Q86Y01; O60630; Q9BS04;
UniGene Hs.372152;
GeneID 1840
KEGG hsa:1840;
Human E3 ubiquitin-protein ligase DTX1 Protein SEQUENCE
SEQUENCE 620 AA; 67368 MW; 3BF3ECE46E25CCD4 CRC64;
MSRPGHGGLM PVNGLGFPPQ NVARVVVWEW LNEHSRWRPY TATVCHHIEN
VLKEDARGSV VLGQVDAQLV PYIIDLQSMH QFRQDTGTMR PVRRNFYDPS
SAPGKGIVWE WENDGGAWTA YDMDICITIQ NAYEKQHPWL DLSSLGFCYL
IYFNSMSQMN RQTRRRRRLR RRLDLAYPLT VGSIPKSQSW PVGASSGQPC
SCQQCLLVNS TRAASNAILA SQRRKAPPAP PLPPPPPPGG PPGALAVRPS
ATFTGAALWA APAAGPAEPA PPPGAPPRSP GAPGGARTPG QNNLNRPGPQ
RTTSVSARAS IPPGVPALPV KNLNGTGPVH PALAGMTGIL LCAAGLPVCL
TRAPKPILHP PPVSKSDVKP VPGVPGVCRK TKKKHLKKSK NPEDVVRRYM
QKVKNPPDED CTICMERLVT ASGYEGVLRH KGVRPELVGR LGRCGHMYHL
LCLVAMYSNG NKDGSLQCPT CKAIYGEKTG TQPPGKMEFH LIPHSLPGFP
DTQTIRIVYD IPTGIQGPEH PNPGKKFTAR GFPRHCYLPN NEKGRKVLRL
LITAWERRLI FTIGTSNTTG ESDTVVWNEI HHKTEFGSNL TGHGYPDASY
LDNVLAELTA QGVSEAAAKA
UCSC uc001tuk.2; human.;

Category

Human DTX1 ELISA Kit