Human DNM1 ELISA Kit

Alternative name
Human DNM ELISA Kit;Human EIEE31 ELISA Kit;Human dynamin 1 ELISA Kit;Human dynamin-1 ELISA Kit;
Catalog
E012504
species
Human
GeneDNM1
SpecificityThis assay has high sensitivity and excellent specificity for detection of DNM1. No significant cross-reactivity or interference between DNM1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DNM1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman DNM1 ELISA Kit allows for the in vitro quantitative determination of DNM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyHuman ELISA Kit
Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This BG DNM1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DNM1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY DNM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DNM1 antibody and an DNM1-HRP conjugate. The assay sample and buffer are incubated together with DNM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DNM1 concentration since DNM1 from samples and DNM1-HRP conjugate compete for the anti-DNM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DNM1 from the sample, fewer sites are left to bind DNM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DNM1 concentration in each sample is interpolated from this standard curve.
Human Dynamin-1 Protein information
Uniprot ID DYN1_HUMAN
Uniprot AC Q05193; A6NLM6; Q5SYX0; Q5SYX2; Q6P3T6; Q86VD2;
UniGene Hs.522413;
GeneID 1759
KEGG hsa:1759;
Human Dynamin-1 Protein SEQUENCE
SEQUENCE 864 AA; 97408 MW; 7FCD8CB572FFEAEF CRC64;
MGNRGMEDLI PLVNRLQDAF SAIGQNADLD LPQIAVVGGQ SAGKSSVLEN
FVGRDFLPRG SGIVTRRPLV LQLVNATTEY AEFLHCKGKK FTDFEEVRLE
IEAETDRVTG TNKGISPVPI NLRVYSPHVL NLTLVDLPGM TKVPVGDQPP
DIEFQIRDML MQFVTKENCL ILAVSPANSD LANSDALKVA KEVDPQGQRT
IGVITKLDLM DEGTDARDVL ENKLLPLRRG YIGVVNRSQK DIDGKKDITA
ALAAERKFFL SHPSYRHLAD RMGTPYLQKV LNQQLTNHIR DTLPGLRNKL
QSQLLSIEKE VEEYKNFRPD DPARKTKALL QMVQQFAVDF EKRIEGSGDQ
IDTYELSGGA RINRIFHERF PFELVKMEFD EKELRREISY AIKNIHGIRT
GLFTPDMAFE TIVKKQVKKI REPCLKCVDM VISELISTVR QCTKKLQQYP
RLREEMERIV TTHIREREGR TKEQVMLLID IELAYMNTNH EDFIGFANAQ
QRSNQMNKKK TSGNQDEILV IRKGWLTINN IGIMKGGSKE YWFVLTAENL
SWYKDDEEKE KKYMLSVDNL KLRDVEKGFM SSKHIFALFN TEQRNVYKDY
RQLELACETQ EEVDSWKASF LRAGVYPERV GDKEKASETE ENGSDSFMHS
MDPQLERQVE TIRNLVDSYM AIVNKTVRDL MPKTIMHLMI NNTKEFIFSE
LLANLYSCGD QNTLMEESAE QAQRRDEMLR MYHALKEALS IIGDINTTTV
STPMPPPVDD SWLQVQSVPA GRRSPTSSPT PQRRAPAVPP ARPGSRGPAP
GPPPAGSALG GAPPVPSRPG ASPDPFGPPP QVPSRPNRAP PGVPSRSGQA
SPSRPESPRP PFDL
UCSC uc064wcg.1; human. [Q05193-1];

Category

Human DNM1 ELISA Kit

Human DNM ELISA Kit;Human EIEE31 ELISA Kit;Human dynamin 1 ELISA Kit;Human dynamin-1 ELISA Kit;