Human DAG1 ELISA Kit

Alternative name
Human Dystrophin-associated glycoprotein 1 ELISA Kit;Human Alpha-DG ELISA Kit;Human Beta-DG ELISA Kit;Human 156DAG ELISA Kit;Human A3a ELISA Kit;Human AGRNR ELISA Kit;Human DAG ELISA Kit;Human MDDGA9 ELISA Kit;Human MDDGC7 ELISA Kit;Human MDDGC9 ELISA Kit;Human dystroglycan 1 ELISA Kit;Human dystroglycan ELISA Kit;Human dystroglycan 1 (dystrophin-associated glycoprotein 1) ELISA Kit;
Catalog
E012580
species
Human
GeneDAG1
SpecificityThis assay has high sensitivity and excellent specificity for detection of DAG1. No significant cross-reactivity or interference between DAG1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DAG1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman DAG1 ELISA Kit allows for the in vitro quantitative determination of DAG1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: DAG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DAG1 antibody and an DAG1-HRP conjugate. The assay sample and buffer are incubated together with DAG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DAG1 concentration since DAG1 from samples and DAG1-HRP conjugate compete for the anti-DAG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DAG1 from the sample, fewer sites are left to bind DAG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DAG1 concentration in each sample is interpolated from this standard curve.
Human Beta-dystroglycan Protein information
Uniprot ID DAG1_HUMAN
Uniprot AC AADEPVTV LTVILDADLT KMTPKQRIDL LHRMRSFSEV ELHNMKLVPV VNNRLFDMSA
UniGene Hs.76111;
GeneID 1605
KEGG hsa:1605;
Human Beta-dystroglycan Protein SEQUENCE
SEQUENCE 895 AA; 97441 MW; 3AF6CBB0DCF91962 CRC64;
MRMSVGLSLL LPLSGRTFLL LLSVVMAQSH WPSEPSEAVR DWENQLEASM
HSVLSDLHEA VPTVVGIPDG TAVVGRSFRV TIPTDLIASS GDIIKVSAAG
KEALPSWLHW DSQSHTLEGL PLDTDKGVHY ISVSATRLGA NGSHIPQTSS
VFSIEVYPED HSELQSVRTA SPDPGEVVSS ACAADEPVTV LTVILDADLT
KMTPKQRIDL LHRMRSFSEV ELHNMKLVPV VNNRLFDMSA FMAGPGNAKK
VVENGALLSW KLGCSLNQNS VPDIHGVEAP AREGAMSAQL GYPVVGWHIA
NKKPPLPKRV RRQIHATPTP VTAIGPPTTA IQEPPSRIVP TPTSPAIAPP
TETMAPPVRD PVPGKPTVTI RTRGAIIQTP TLGPIQPTRV SEAGTTVPGQ
IRPTMTIPGY VEPTAVATPP TTTTKKPRVS TPKPATPSTD STTTTTRRPT
KKPRTPRPVP RVTTKVSITR LETASPPTRI RTTTSGVPRG GEPNQRPELK
NHIDRVDAWV GTYFEVKIPS DTFYDHEDTT TDKLKLTLKL REQQLVGEKS
WVQFNSNSQL MYGLPDSSHV GKHEYFMHAT DKGGLSAVDA FEIHVHRRPQ
GDRAPARFKA KFVGDPALVL NDIHKKIALV KKLAFAFGDR NCSTITLQNI
TRGSIVVEWT NNTLPLEPCP KEQIAGLSRR IAEDDGKPRP AFSNALEPDF
KATSITVTGS GSCRHLQFIP VVPPRRVPSE APPTEVPDRD PEKSSEDDVY
LHTVIPAVVV AAILLIAGII AMICYRKKRK GKLTLEDQAT FIKKGVPIIF
ADELDDSKPP PSSSMPLILQ EEKAPLPPPE YPNQSVPETT PLNQDTMGEY
TPLRDEDPNA PPYQPPPPFT APMEGKGSRP KNMTPYRSPP PYVPP
UCSC uc003cxc.5; human.;

Category

Human DAG1 ELISA Kit